Cell-Free Synthesis of the Mitochondrial ADP/ATP Carrier Protein of Neurospora crassa
Article first published online: 28 JUN 2008
European Journal of Biochemistry
Volume 99, Issue 2, pages 247–252, September 1979
How to Cite
ZIMMERMAN, R., PALUCH, U., SPRINZL, M. and NEUPERT, W. (1979), Cell-Free Synthesis of the Mitochondrial ADP/ATP Carrier Protein of Neurospora crassa. European Journal of Biochemistry, 99: 247–252. doi: 10.1111/j.1432-1033.1979.tb13251.x
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- (Received May 9, 1979)
ADP/ATP carrier protein was synthesized in heterologous cell-free systems programmed with Neurospora poly(A)-containing RNA and homologous cell-free systems from Neurospora. The apparent molecular weight of the product obtained in vitro was the same as that of the authentic mitochondrial protein. The primary translation product obtained in reticulocyte lysates starts with formylmethionine when formylated initiator methionyl-tRNA (fMet-tRNAfMet) was present. The product synthesized in vitro was released from the ribosomes into the postribosomal supernatant.
The evidence presented indicates that the ADP/ATP carrier is synthesized as a polypeptide with the same molecular weight as the mature monomeric protein and does not carry an additional sequence.