Cell-Free Synthesis of the Mitochondrial ADP/ATP Carrier Protein of Neurospora crassa

Authors

  • Richard ZIMMERMAN,

    1. Physiologisch-Chemisches Institut der Georg-August-Universität zu Göttingen, Humboldtallee 7, D-3400 Göttingen, Federal Republic of Germany
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  • Ulrich PALUCH,

    1. Physiologisch-Chemisches Institut der Georg-August-Universität zu Göttingen, Humboldtallee 7, D-3400 Göttingen, Federal Republic of Germany
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  • Matthias SPRINZL,

    1. Abteilung, Chemie, Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Straße 3, D-3400 Göttingen, Federal Republic of Germany
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  • Walter NEUPERT

    1. Physiologisch-Chemisches Institut der Georg-August-Universität zu Göttingen, Humboldtallee 7, D-3400 Göttingen, Federal Republic of Germany
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Abstract

ADP/ATP carrier protein was synthesized in heterologous cell-free systems programmed with Neurospora poly(A)-containing RNA and homologous cell-free systems from Neurospora. The apparent molecular weight of the product obtained in vitro was the same as that of the authentic mitochondrial protein. The primary translation product obtained in reticulocyte lysates starts with formylmethionine when formylated initiator methionyl-tRNA (fMet-tRNAfMet) was present. The product synthesized in vitro was released from the ribosomes into the postribosomal supernatant.

The evidence presented indicates that the ADP/ATP carrier is synthesized as a polypeptide with the same molecular weight as the mature monomeric protein and does not carry an additional sequence.

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