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Glycogen Synthase from Rabbit Skeletal Muscle

Amino Acid Sequence at the Sites Phosphorylated by Glycogen Synthase Kinase-3, and Extension of the N-Terminal Sequence Containing the Site Phosphorylated by Phosphorylase Kinase

  1. Dennis B. RYLATT1,
  2. Alastair AITKEN2,
  3. Terence BILHAM2,
  4. Gerard D. CONDON2,
  5. Noor EMBI2,
  6. Philip COHEN2

Article first published online: 3 MAR 2005

DOI: 10.1111/j.1432-1033.1980.tb06060.x

Issue

European Journal of Biochemistry

European Journal of Biochemistry

Volume 107, Issue 2, pages 529–537, June 1980

Additional Information

How to Cite

RYLATT, D. B., AITKEN, A., BILHAM, T., CONDON, G. D., EMBI, N. and COHEN, P. (1980), Glycogen Synthase from Rabbit Skeletal Muscle. European Journal of Biochemistry, 107: 529–537. doi: 10.1111/j.1432-1033.1980.tb06060.x

Author Information

  1. 1

    Department of Microbiology, John Curtin School of Medical Research, The Australian National University, P.O. Box 334, Canberra, Australian Capital Territory, Australia 2601

  2. 2

    Departmcnt of Biochemistry, University of Dundee, Medical Sciences Institute, Dundee, Great Britain, DDI 4flN

Publication History

  1. Issue published online: 3 MAR 2005
  2. Article first published online: 3 MAR 2005
  3. (Received December 18, 1979)

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Glycogen synthase kinase-3 phosphorylates three serine residues on glycogen synthase (sites 3a, 3b and 3c) which are all located in the same nine-amino-acid segment of the polypeptide chain. The sequence in this region is: Arg-Tyr-Pro-Arg-Pro-Ala-Ser(P)-Val-Pro-Pro-Ser(P)-Pro-Ser-Leu-Ser(P)-Arg-. These serine residues are distinct from the sites phosphorylated preferentially by cyclic-AMP-dependent protein kinase (sites 1a and 1b) and phosphorylase kinase (site 2).

The N-terminal sequence of glycogen synthase containing the serine residue phosphorylated by phosphorylase kinase has been extended. The sequence in this region is: Pro-Leu-Ser-Arg-Thr-Leu-Ser(P)-Val-Ser-Ser-Leu-Pro-Gly-Leu-Glu-Asp-Trp-Glu-Asp-Glu-Phe-Asp-Leu-Glu-Asn-Ser-Val-Leu-Phe-(Asx2,Glx2,Ala2,Val2,Lys)-. The similarity to the N-terminal sequence of phosphorylase is confined to the immediate vicinity of the phosphorylation site (residues 4–15).

The relationship of glycogen synthase kinase-3 to glycogen synthase kinases that have been described by other laboratories is discussed.

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