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Abstract

  1. Top of page
  2. Abstract
  3. REFERENCES

Protein phosphatase-2B was purified from extracts of rabbit skeletal muscle by a procedure that involved fractionation with ammonium sulphate, chromatography on DEAE-Sepharose, fractionation with poly(ethylene glycol), gel filtration on Sephadex G-200 (Mr= 98000 ± 4000), chromatography on Affi-Gel Blue and affinity chromatography on calmodulin-Sepharose. The enzyme was purified 35000-fold in senven days with an overall yield of 0.5%.

The α-subunit of phosphorylase kinase, protein phosphatase inhibitor-1 and the myosin P-light chain from rabbit skeletal muscle were dephosphorylated by protein phosphatase-2B with similar kinetic constants. The α-subunit of phosphorylase kinase was dephosphorylated at least 100-fold more rapidly tha the β-subunit, while glycogen phosphorylase, glycogen phosphorylase, glycogen synthase, histones H1 and H2B, ATP-citrate lyase, acetyl-CoA carboxylase, l-pyruvate kinase and protein synthesis initiation factor eIF-2 were not dephosporylated at significant rates.

Protein phosphatase-2B became activated 10-fold by calmodulin (A0.5= 6nM) after chromatography on DEAE-Sepharose and this degree of activation was maintained throughout the remainder of the purification. Calmodulin increased the Vmax of the reaction without altering the Km for inhibitor-1.

The activity of protein phosphatase-2B was completely dependent on Ca2+ in the presence or absence of calmodulin. Half-maximal activation was observed at 1.0 μM Ca2+ in the presence, of 0.03 μM calmodulin. Protein phosphatase-2B was inhibited completely by trifluoperazine; halfmaximal inhibition occurred at 45 μM in the presence of 0.03 μM calmodulin.

The metabolic role of protein phosphatase-2B in vivo is discussed in the light of the observation that this enzyme is probably identical to a major calmodulin-binding protein of neural tissue termed calcineurin or CaAM-BP80 [Stewart, A. A., Ingebritsen, T. S., Manalan, A., Klee, C. B., and Cohen, P. (1982) FEBS lett. 137, 80–84].

Enzymes
 

Glycogen phosphorylase (EC 2.4.1.1)

 

glycogen synthase (EC 2.4.1.11)

 

phosphorylase kinase (EC 2.7.1.38)

 

ATP-citrate lyase (EC 4.1.3.8)

 

acetyl-CoA carboxylase (EC 6.4.1.2)

 

pyruvate kinase (EC 2.7.1.40)

 

urease (EC 3.5.1.5)

 

alcohol dehdrogenase (EC 1.1.1.1)

 

peroxidase (EC 1.11.1.7)

 

carbonic anhydrase (EC 4.2.1.1)

 

protein phosphatase (EC 3.1.3.-)

REFERENCES

  1. Top of page
  2. Abstract
  3. REFERENCES
  • 1
    Ingebritsen, T. S. & Cohen, P. (1983) Eur. J. Biochem. 132, 255261.
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  • 3
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