Role of phosphate in the regulation of the Pasteur effect in Saccharomyces cerevisiae

Authors

  • Rosario LAGUNAS,

    1. Instituto de Enzimología y Patología Molecular del Consejo Superior de Investigaciones Cientificas, Facultad de Medicina de la Universidad Autónoma, Canto Blanco, Madrid-34, Spain
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  • Carlos GANCEDO

    1. Instituto de Enzimología y Patología Molecular del Consejo Superior de Investigaciones Cientificas, Facultad de Medicina de la Universidad Autónoma, Canto Blanco, Madrid-34, Spain
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Abstract

The occurrence of the Pasteur effect in Saccharomyces cerevisiae in several conditions has been examined. In these conditions measurements of a series of metabolites potentially involved in the regulation of the effect were performed. These included, among others, adenine nucleotides, citrate, fructose 2,6-bisphosphate and phosphate. Only phosphate changed in a consistent way, increasing in anaerobiosis when the Pasteur effect occurred. It is concluded that, with the available data, only phosphate may be considered as a regulator of the Pasteur effect in this microorganism.

Enzymes
 

Phosphofructokinase (EC 2.7.1.11)

 

hexokinase (EC 2.7.1.1)

 

isocitrate dehydrogenase (EC 1.1.1.41)

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