Catabolite repression in yeasts is not associated with low levels of cAMP
Article first published online: 3 MAR 2005
European Journal of Biochemistry
Volume 141, Issue 1, pages 195–198, May 1984
How to Cite
ERASO, P. and GANCEDO, J. M. (1984), Catabolite repression in yeasts is not associated with low levels of cAMP. European Journal of Biochemistry, 141: 195–198. doi: 10.1111/j.1432-1033.1984.tb08174.x
- Issue published online: 3 MAR 2005
- Article first published online: 3 MAR 2005
- (Received November 14, 1983) – EJB 83 1227
The relationship between levels of cAMP and catabolite repression in yeasts has been investigated. Strains of Saccharomyces cerevisiae, Schizosaccharomyces pombe and Kluyieromyces fragilis were used. The yeasts were grown on different carbon sources to attain various degrees of repression. Galactose repressed as much as glucose, while maltose was less effective. Full derepression was achieved with ethanol.
The enzymes tested were fructose-bisphosphatase, malate dehydrogenase, glutamate dehydrogenase (NAD dependent), cytochrome oxidase and isocitrate lyase (this last enzyme was found to be absent in Schizosaccharomyces).
The levels of cAMP were 2–3 times higher in the repressed conditions than in the derepressed ones. It is therefore concluded that in yeasts catabolite repression is not mediated by a lowering of the intracellular concentration of cAMP.