Human placental neuraminidase

Activation, stabilization and association with β-galactosidase and its ‘protective’ protein

Authors

  • Frans W. VERHEIJEN,

    Corresponding author
    1. Department of Cell Biology and Genetics, Erasmus University Rotterdam; and Institute of Neurological Sciences, Medical School, University of Siena
      Corresponds to F. W. Verheijen, Afdeling Celbiologie en Genetica, Erasmus Universiteit Rotterdam, P.O. Box 1738, NL-3000-DR Rotterdam, The Netherlands
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  • Silvia PALMERI,

    1. Department of Cell Biology and Genetics, Erasmus University Rotterdam; and Institute of Neurological Sciences, Medical School, University of Siena
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  • André T. HOOGEVEEN,

    1. Department of Cell Biology and Genetics, Erasmus University Rotterdam; and Institute of Neurological Sciences, Medical School, University of Siena
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  • Hans GALJAARD

    1. Department of Cell Biology and Genetics, Erasmus University Rotterdam; and Institute of Neurological Sciences, Medical School, University of Siena
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Corresponds to F. W. Verheijen, Afdeling Celbiologie en Genetica, Erasmus Universiteit Rotterdam, P.O. Box 1738, NL-3000-DR Rotterdam, The Netherlands

Abstract

Supernatant of homogenized human placenta hardly contains lysosomal neuraminidase activity. It is, however, possible to generate remarkably high activity by concentration of a partially purified glycoprotein fraction. This activity is labile to dilution, but can be stabilized by incubation at 37°C and acid pH.

Using β-galactosidase specific affinity chromatography and immunotitration, we show that the activated and stabilized human lysosomal neuraminidase exists in a complex with β-galactosidase. Sucrose density gradient centrifugation experiments demonstrate that the neuraminidase activity is exclusively present in a high density multimeric form of β-galactosidase.

The formation of multimeric forms of β-galactosidase is known to require a 32000-Mr‘protective’ protein. Monospecific antibodies against this ‘protective’ protein were purified from a conventional antiserum containing a mixture of antibodies against the 64000-Mrβ-galactosidase protein and against the 32000-Mr‘protective’ protein, using a nitrocellulose blot immunoaffinity purification procedure. Immunotitration experiments with these antibodies show that the 32000-Mr‘protective’ protein is present both in association with the β-galactosidase multimer and with the high-density multimeric form together with neuraminidase.

Our data further suggest that association of the 32000-Mr‘protective’ protein and another yet unidentified subunit is essential for the catalytic activity of lysosomal neuraminidase. These results explain the absence of neuraminidase activity in the autosomal recessive human lysosomal storage disorder galactosialidosis, where the 32000-Mr‘protective’ protein is known to be absent.

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