Primary structure of a new tetraantennary glycan of the N-acetyllactosaminic type isolated from human factor VIII/von Willebrand factor

Authors


Correspondence to J. Montreuil, Laboratoire de Chimie Biologique (Unité Associée au CNRS no.217), Université des Sciences et Techniques de Lille-Flandres-Artois, F-59655 Villeneuve d'Ascq Cedex, France

Abstract

N-Glycosidically linked glycopeptides released by mild alkaline treatment of human factor VIII/von Willebrand factor (FVIII/vWF) were fractionated by serial affinity chromatography on columns of Sepharose linked to concanavalin A (ConA) and Lens culinaris agglutinin (LCA). The fraction which is not retained on ConA-Sepharose, but eluted from LCA-Sepharose contains a pure minor glycopeptide which was structurally analysed. Based on the results of methylation analysis and 500-MHz 1H-NMR spectroscopy, the following structure is proposed:

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Abbreviations
ConA

concanavalin A

LCA

Lens culinaris agglutinin

NeuAc

N-acetyl-neuraminic acid

Gal

d-galactose

Man

d-mannose

GlcNAc

N-acetyl-d-glucosamine

Fuc

l-fucose

Asn

L-asparagine

FVIII/vWF

factor VIII/von Willebrand factor

MeGlc

methyl α-glucoside

Ancillary