Rainbow trout protamines

Amino acid sequences of six distinct proteins from a single testis

Authors

  • Donald J. McKAY,

    1. Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Calgary, Alberta
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  • Bernard S. RENAUX,

    1. Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Calgary, Alberta
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  • Gordon H. DIXON

    Corresponding author
    1. Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Calgary, Alberta
      Correspondence to G. H. Dixon, Department of Medical Biochemistry, University of Calgary Health Sciences Centre, 3330 Hospital Drive, Calgary, Alberta, Canada T2N 4NI
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Correspondence to G. H. Dixon, Department of Medical Biochemistry, University of Calgary Health Sciences Centre, 3330 Hospital Drive, Calgary, Alberta, Canada T2N 4NI

Abstract

All the protamines present in detectable amounts in a single mature testis from rainbow trout have been purified to homogeneity using acid extraction, gel filtration chromatography on Bio-Gel P-10, ion-exchange chromatography on carboxymethylcellulose and reverse-phase high-pressure liquid chromatography. Each of the six purified protamines was completely sequenced using automated gas-phase Edman degradation. Each protamine is two-thirds arginine and also contains proline, serine, valine and glycine. Three protamines also contain alanine while two contain isoleucine. Four of the protamines have 32 amino acids while the remaining two have 30. The six protamines have been classified into three families on the basis of their amino acid sequences.

Abbreviations
HPLC

high-pressure liquid chromatography

 

CM-cellulose carboxymethylcellulose

Ancillary