This paper is dedicated to the memory of our colleague Jordi Cortadas (†1985) who first demonstrated the possibility of fractionatign main-band DNAs of warm-blooded vertebrates by Cs2SO4/BAMD centrifugation.
Gene distribution and nucleotide sequence organization in the mouse genome
Article first published online: 3 MAR 2005
European Journal of Biochemistry
Volume 160, Issue 3, pages 469–478, November 1986
How to Cite
SALINAS, J., ZERIAL, M., FILIPSKI, J. and BERNARDI, G. (1986), Gene distribution and nucleotide sequence organization in the mouse genome. European Journal of Biochemistry, 160: 469–478. doi: 10.1111/j.1432-1033.1986.tb10063.x
- Issue published online: 3 MAR 2005
- Article first published online: 3 MAR 2005
- (Received April 2, 1986) – EJB 86 0356
Mouse DNA was fractionated by preparative centrifugation in density gradients of Cs2SO4 containing 3,6-bis(acetatomercurimethyl)dioxane (BAMD). The effects of temperature, BAMD/nucleotide molar ratio and solvent on the fractionation were explored. The fractions so obtained were investigated by analytical centrifugation in CsCl density gradient and by hybridization with a number of gene probes. These approaches led (a) to the definition of satisfactory conditions for the rapid fractionation of mouse DNA; (b) to the localization of a number of genes in mouse DNA fractions; and (c) to a better understanding of the mosaic organization of the mouse genome and, more specifically, to a better estimate of both the intermolecular and intramolecular compositional heterogeneity of mouse DNA in the (75–150) × 103-base size range.