Early chick embryos at the age of 22 h (neurula stage) and 48 h (20–25 somite stage) were explanted from eggs and cultured in vitro in the presence of radioactive sugar precursors. Metabolically labelled glycosphingolipids were isolated. Amongst these, neutral and acidic components, the latter including sulfatide and gangliosides, were identified. Cleavage by exoglycosidases, as well as immunostaining with antibodies on thin-layer plates, showed that at both embryonic stages glycosphingolipids were synthesized that belong to the globo series (globoside, Forssman glycolipid), the lacto series (lactoneotetraosylceramide, nLc4Cer, and two nLc4Cer-based gangliosides, a monosialo and a disialo species), and the ganglio series (ganglioside Gtet1a and higher sialylated derivatives).
If you can't find a tool you're looking for, please click the link at the top of the page to "Go to old article view". Alternatively, view our Knowledge Base articles for additional help. Your feedback is important to us, so please let us know if you have comments or ideas for improvement.
ceramide pentahexoside. Gangliosides are designated according to their constituents by using short-hand notations as detailed earlier  [see also Eur. J. Biochem. 79, 11–21 (1979)]: Ggal1
highperformance thin-layer chromatography
α-Galactosidase (EC 188.8.131.52)
β-galactosidase (EC 184.108.40.206)
α-N-acetylgalactosaminidase (EC 220.127.116.11)
β-N-acetylhexosaminidase (EC 18.104.22.168)
neuraminate glycohydrolase or sialidase (EC 22.214.171.124)