SEARCH

SEARCH BY CITATION

Abstract

  1. Top of page
  2. Abstract
  3. REFERENCES

A 9000-Mr protein isolated from a 60% ethanolic extract of soybean (Glycine max) seeds has been characterized and fully sequenced. The protein consists of 80 amino acid residues with four disulfide bonds. It contains a large number of hydrophobic residues and lacks methionine, phenylalanine, tryptophan, lysine and histidine residues. The protein readily crystallizes from water but is quite soluble in aqueous organic solvents like 95% 1-propanol. It aggregates to form large molecules (above 80 kDa) under ordinary denaturing conditions, such as 6 M guanidine · HCl and 8 M urea. Sequence analysis showed that the amino-terminal four-fifths is extremely hydrophobic and most of the acidic residues exist as their amide forms, and only the carboxyl-terminal short segment is rather hydrophilic. A computer search for homology detected an unexpected similarity of this protein to rat prolactin; however, its significance could not be assessed and this protein appears to represent a hitherto unknown protein family. Although no biochemical activity could be detected, the existence in relatively high abundance (approx. 200 mg from 1 kg seeds) of this novel protein may suggest its physiological significance in the plant.

Enzymes
 

α-Chymotrypsin (EC 3.4.21.1)

 

trypsin (EC 3.4.21.4)

 

elastase (EC 3.4.21.36)

 

papain (EC 3.4.22.2)

 

triacylglycerol lipase (EC 3.1.1.3)

 

α-amylase (EC 3.2.1.1)

REFERENCES

  1. Top of page
  2. Abstract
  3. REFERENCES
  • 1
    Van Etten, C. H., Nelson, H. C. & Peters, J. E. (1965) Phytochemistry 4, 467473.
  • 2
    Teeter, N. M., Mazer, J. A. & L'Italien, J. J. (1981) Biochemistry 20, 54375443.
  • 3
    Hendrickson, W. A. & Teeter, N. M. (1981) Nature (Lond.) 290, 107113.
  • 4
    Garcia-Olmedo, F. & Garcia-Faure, R. (1969) Lebensm. Wiss: Technol. 2, 9496.
  • 5
    Shewry, P. R., Lafiandra, D., Salcedo, G., Aragnoncillo, C., Garcia-Olmedo, F., Lew, E. J.-L., Dietler, M. D. & Kasarda, D. D. (1984) FEBS Lett. 175, 359363.
  • 6
    Ui, N. (1979) Anal. Biochem. 97, 6571.
  • 7
    Swergold, G. D. & Rubin, C. S. (1983) Anal. Biochem. 131, 295300.
  • 8
    Hirs, C. H. W. (1967) Methods Enzymol. 11, 5962.
  • 9
    Ellman, G. L. (1959) Arch. Biochem. Biophys. 82, 7077.
  • 10
    Moore, S. (1972) in Chemistry and biology of petides (Meienhofer, J., ed.) pp. 629653, Ann Abor Publishers, Michigan .
  • 11
    Edman, P. & Begg, G. (1967) Eur. J. Biochem. 1, 8091.
  • 12
    Brauer, A. W., Margolies, M. N. & Harber, E. (1975) Biochemistry 14, 30293035.
  • 13
    Tarr, G. E., Beecher, J. F., Bell, M. & McKean, D. J. (1978) Anal. Biochem. 84, 622627.
  • 14
    Hirs, C. H. W. (1967) Methods Enzymol. 11, 199203.
  • 15
    Odani, S. & Ikenaka, T. (1977) J. Biochem. (Tokyo) 82, 15131522.
  • 16
    Arnon, R. (1970) Methods Enzymol. 19, 226252.
  • 17
    Wahlefeld, A. W. (1984) in Methods in enzymatic analysis, 3rd edn (Bergmeyer, H. U., Bergmeyer, J., Grassl, M. & Moss, D. W., eds) vol. 4, pp. 161167, Verlag Chemie, Weinheim .
  • 18
    Junge, W. (1984) in Methods in enzymatic analysis, 3rd edn (Bergmeyer, H. U., Bergmeyer, J., Grassl, M. & Moss, D. W., eds) vol. 4, pp. 1525, Verlag Chemie, Weinheim .
  • 19
    Aoyagi, Y., Takahashi, Y., Odani, S., Ogata, K., Ono, T. & Ichida, F. (1982) J. Biol. Chem. 257, 95669569.
  • 20
    Osawa, T. & Matsumoto, I. (1972) Methods Enzymol. 28, 323328.
  • 21
    Davis, B. J. (1964) Ann. New York Acad. Sci. 121, 404442.
  • 22
    Reisfeld, R. A., Lewis, U. J. & Williams, D. J. (1962) Nature (Lond.) 195, 281283.
  • 23
    Narita, K., Matsuo, H. & Nakajima, T. (1975) in Protein sequence determination (Needleman, S. B., ed.) pp. 30103, Springer-Verlag, Berlin .
  • 24
    Chou, P. Y. & Fassman, G. D. (1978) Adv. Enzymol. 47, 145148.
  • 25
    Kyte, J. & Doolittle, R. F. (1982) J. Mol. Biol. 157, 105132.
  • 26
    Waterman, M. S., Smith, T. F. & Beyer, W. A. (1976) Adv. Math. 20, 367387.
  • 27
    Cooke, N. E., Coit, D., Weiner, R. I., Baxter, J. D. & Martial, J. A. (1980) J. Biol. Chem. 255, 65026510.
  • 28
    Dayhoff, M. O., Barker, W. C. & Hunt, T. L. (1983) Methods Enzymol. 91, 524545.
  • 29
    Borgstroem, B. & Erlanson, C. (1978) Gastroenterology 75, 382386.
  • 30
    Gargouri, Y., Julien, R., Sugihara, A., Verger, R. & Sarda, L. (1984) Biochim. Biophys. Acta 795, 326331.
  • 31
    Gasperi-Campani, A., Barbieri, L., Lorenzioni, E. & Stirpe, F. (1977) FEBS Lett. 76, 173176.
  • 32
    Gatehause, J. A., Gilroy, J., Hoque, M. S. & Croy, R. R. D. (1985) Biochem. J. 225, 239247.