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Nucleotide sequence of a cellulase gene of Bacillus subtilis

  1. Akira NAKAMURA,
  2. Takeshi UOZUMI,
  3. Teruhiko BEPPU*

Article first published online: 3 MAR 2005

DOI: 10.1111/j.1432-1033.1987.tb11060.x

Issue

European Journal of Biochemistry

European Journal of Biochemistry

Volume 164, Issue 2, pages 317–320, April 1987

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How to Cite

NAKAMURA, A., UOZUMI, T. and BEPPU, T. (1987), Nucleotide sequence of a cellulase gene of Bacillus subtilis. European Journal of Biochemistry, 164: 317–320. doi: 10.1111/j.1432-1033.1987.tb11060.x

Author Information

  1. Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo

*Correspondence to T. Beppu, Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Bunkyoku, 113, Japan.

Publication History

  1. Issue published online: 3 MAR 2005
  2. Article first published online: 3 MAR 2005
  3. (Received October 10/December 23, 1986) – EJB 86 1090

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The nucleotide sequence of an endolytic cellulase gene of Bacillus subtilis was determined and compared with the NH2-terminal amino acid sequence of the purified enzyme. The mature protein appeared to be extended by a signal sequence of 36 amino acids. The putative AUG initiation codon was preceded by a σ43-type promoter of B. subtilis and an AAGGAGG sequence, typical of procaryotic ribosomal binding sites. Partial homology of amino acid sequences was dound between B. subtilis cellulase and an alkalophilic Bacillus cellulase.

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