You have full text access to this OnlineOpen article

A human embryonic lung fibroblast with a high density of muscarinic acetylcholine receptors

  1. Claudine ANDRÉ1,
  2. Stefano MARULLO1,
  3. André CONVENTS3,
  4. Bao Zhang LÜ2,
  5. Jean Gérard GUILLET2,
  6. Johan HOEBEKE2,
  7. A. Donny STROSBERG2,*

Article first published online: 3 MAR 2005

DOI: 10.1111/j.1432-1033.1988.tb13804.x

Issue

European Journal of Biochemistry

European Journal of Biochemistry

Volume 171, Issue 1-2, pages 401–407, January 1988

Additional Information

How to Cite

ANDRÉ, C., MARULLO, S., CONVENTS, A., LÜ, B. Z., GUILLET, J. G., HOEBEKE, J. and STROSBERG, A. D. (1988), A human embryonic lung fibroblast with a high density of muscarinic acetylcholine receptors. European Journal of Biochemistry, 171: 401–407. doi: 10.1111/j.1432-1033.1988.tb13804.x

Author Information

  1. 1

    Laboratoire de Biochimie Cellulaire, Collège de France, Paris

  2. 2

    Département de Biotechnologie, Institut Pasteur, Paris

  3. 3

    Laboratorium voor Proteine Scheikunde, Instituut voor Molekulaire Biologie, Sint Genesius Rode

*Correspondence to A. D. Strosberg, Département de Biotechnologie, Institut Pasteur, 28 rue du Dr Roux, F-75724 Paris Cédex 15, France

Publication History

  1. Issue published online: 3 MAR 2005
  2. Article first published online: 3 MAR 2005
  3. (Received August S/September 30, 1987) – EJB 87 0908

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (721K)

Binding studies with the radiolabeled muscarinic antagonists dexetimide, quinuclidinyl benzilate and N-methylscopolamine showed that the human embryonic lung fibroblast CCL137 possesses approximately 2 × 105 muscarinic receptors/cell, i.e. 2.1 pmol/mg membrane protein. These receptors showed a marked stereoselectivity towards dexetimide and levetimide and only low affinity for another antagonist, pirenzepine. The muscarinic agonist carbamylcholine inhibited forskolin-stimulated adenylate cyclase and induced phosphatidylinositide turnover in the intact cells. Both effects were inhibited by the muscarinic antagonist atropine. Affinity labeling with tritiated propylbenzylcholine mustard revealed a protein of 72 kDa. Finally, down-regulation of the membrane receptors following prolonged treatment with the agonist carbamylcholine was assessed by means of the hydrophilic antagonist N-methylscopolamine.

View Full Article (HTML) Get PDF (721K)