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Cloning and expression of cDNA for human vascular anticoagulant, a Ca2+-dependent phospholipid-binding protein
Article first published online: 3 MAR 2005
DOI: 10.1111/j.1432-1033.1988.tb14139.x
1742-4658/asset/cover.gif?v=1&s=fc98a9fc84ccc5e1b83463cabb40f397544364eb "European Journal of Biochemistry")
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How to Cite
MAURER-FOGY, I., REUTELINGSPERGER, C. P. M., PIETERS, J., BODO, G., STRATOWA, C. and HAUPTMANN, R. (1988), Cloning and expression of cDNA for human vascular anticoagulant, a Ca2+-dependent phospholipid-binding protein. European Journal of Biochemistry, 174: 585–592. doi: 10.1111/j.1432-1033.1988.tb14139.x
Publication History
- Issue published online: 3 MAR 2005
- Article first published online: 3 MAR 2005
- (Received February 26, 1988) – EJB 88 0236
- Abstract
- Article
- References
- Cited By
Based on sequence information from tryptic peptides an almost full-size cDNA coding for the human vascular anticoagulant was isolated from a placental cDNA library and sequenced. The coding region was cloned into an Escherichia coli expression vector and the protein expressed at high levels. The recombinant protein was purified and found to be indistinguishable from its natural counterpart in several biological assays.