Note. The novel nucleotide sequence data published here has been deposited with the EMBL sequence data banks and is available under accession number X61144.
Identification and characterization of the lantibiotic nisin Z, a natural nisin variant
Article first published online: 3 MAR 2005
European Journal of Biochemistry
Volume 201, Issue 3, pages 581–584, November 1991
How to Cite
MULDERS, J. W. M., BOERRIGTER, I. J., ROLLEMA, H. S., SIEZEN, R. J. and de VOS, W. M. (1991), Identification and characterization of the lantibiotic nisin Z, a natural nisin variant. European Journal of Biochemistry, 201: 581–584. doi: 10.1111/j.1432-1033.1991.tb16317.x
The novel amino acid sequence data published here has been deposited with the EMBL sequence data bank.
- Issue published online: 3 MAR 2005
- Article first published online: 3 MAR 2005
- (Received June 3, 1991) – EJB 91 0713
Lactococcus lactis strain NIZO 22186 produces an extracellular, lanthinine-containing 3.5-kDa polypeptide with antimicrobial activity. Its retention time on reversed-phase (RP) HPLC and its amino acid composition showed high similarities but no complete identity to nisin. The gene for this lantibiotic, designated nisZ, has been cloned and its nucleotide sequence was found to be identical to that of the precursor nisin gene apart from a single mutation resulting in the substitution His27Asn in the mature polypeptide. NMR studies of the natural nisin variant, which has been designated nisin Z, confirmed the His27Asn substitution and idicated that it has a similar structure to nisin.