Identification and characterization of the lantibiotic nisin Z, a natural nisin variant

Authors

  • John W. M. MULDERS,

    1. Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede, The Netherlands
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  • Ingrid J. BOERRIGTER,

    1. Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede, The Netherlands
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  • Harry S. ROLLEMA,

    1. Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede, The Netherlands
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  • Roland J. SIEZEN,

    1. Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede, The Netherlands
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  • Willem M. de VOS

    Corresponding author
    1. Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede, The Netherlands
      Correspondence to W. M. de Vos, Netherlands Institute for Dairy Research (NIZO), P. O. Box 20, NL-6710 BA Ede, The Netherlands
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  • Note. The novel nucleotide sequence data published here has been deposited with the EMBL sequence data banks and is available under accession number X61144.

    The novel amino acid sequence data published here has been deposited with the EMBL sequence data bank.

Correspondence to W. M. de Vos, Netherlands Institute for Dairy Research (NIZO), P. O. Box 20, NL-6710 BA Ede, The Netherlands

Abstract

Lactococcus lactis strain NIZO 22186 produces an extracellular, lanthinine-containing 3.5-kDa polypeptide with antimicrobial activity. Its retention time on reversed-phase (RP) HPLC and its amino acid composition showed high similarities but no complete identity to nisin. The gene for this lantibiotic, designated nisZ, has been cloned and its nucleotide sequence was found to be identical to that of the precursor nisin gene apart from a single mutation resulting in the substitution His27Asn in the mature polypeptide. NMR studies of the natural nisin variant, which has been designated nisin Z, confirmed the His27Asn substitution and idicated that it has a similar structure to nisin.

Abbreviations
RP

reversed phase

Ile

isoleucine

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