Note. The novel amino acid sequence data published here have been submitted to the EMBL sequence data bank(s) (accession No. P80144).
A rat cerebellar protein containing the cdc10/SWI6 motif
Article first published online: 3 MAR 2005
European Journal of Biochemistry
Volume 207, Issue 2, pages 615–620, July 1992
How to Cite
TAOKA, M., YAMAKUNI, T., SONG, S.-Y., YAMAKAWA, Y., SETA, K., OKUYAMA, T. and ISOBE, T. (1992), A rat cerebellar protein containing the cdc10/SWI6 motif. European Journal of Biochemistry, 207: 615–620. doi: 10.1111/j.1432-1033.1992.tb17088.x
- Issue published online: 3 MAR 2005
- Article first published online: 3 MAR 2005
- (Received February 13/April 21, 1992) – EJB 92 0193
Systematic analysis of soluble proteins in developing rat cerebellum by an automated two-dimensional liquid-chromatography system detected a number of proteins which increased transiently during the initial stage of postnatal development. One of the proteins, V-1 was isolated using a liquid-chromatography system, and its amino acid sequence was determined by analysis of the purified protein. The sequence showed that the V-1 protein consisted of 117 amino acids with an acetylated N-terminus, and has 2.5 internal sequence repeats of 33 amino acids. Computer retrieval of the sequence indicated that the repeated sequences have a structural characteristics of the cdc10/SWI6 motif, which is found in a series of proteins, including those involved in cell-cycle control and cell-fate determination in yeast, Drosophila melanogaster and Caenorhabditis elegans. The structure of V-1, coupled with its controlled expression in early postnatal development, implies a potential role for V-1 in cerebellar morphogenesis.