Note. This article is dedicated to the memory of Thomas Emery.
Iron release from ferrisiderophores
A multi-step mechanism involving a NADH/FMN oxidoreductase and a chemical reduction by FMNH2
Article first published online: 3 MAR 2005
European Journal of Biochemistry
Volume 209, Issue 2, pages 621–627, October 1992
How to Cite
HALLÉ, F. and MEYER, J.-M. (1992), Iron release from ferrisiderophores. European Journal of Biochemistry, 209: 621–627. doi: 10.1111/j.1432-1033.1992.tb17328.x
- Issue published online: 3 MAR 2005
- Article first published online: 3 MAR 2005
- (Received May 21, 1992) – EJB 92 0710
Release of iron from various ferrisiderophores (ferripyoverdines, ferrioxamines B and E, ferricrocin, ferrichrome A, ferrienterobactin and its analog ferric N,N′,N”-tri(1,3,5-Tris) 2,3-dihydroxybenzoylaminomethylbenzene) was obtained through an enzymic reduction of iron, involving NADH, FMN and the ferripyoverdine reductase of Pseudomonas aeruginosa PAO1. The iron released from the same complexes was also obtained through chemical reduction of iron involving FMNH2. Evidence is given that the enzymic process acts through a FMNH2 reduction; the P. aeruginosa enzyme, purified according to its ferripyoverdine-reductase activity [Hallé, F. & Meyer, J. M., Eur. J. Biochem. 209, 613–620], functions as a NADH:FMN oxidoreductase, the FMNH2 produced being able to chemically reduce the iron complexed by siderophores. The general occurence of such a multi-step mechanism, which denies the existence of specific ferrisiderophore reductases, is discussed.