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Abstract

  1. Top of page
  2. Abstract
  3. REFERENCES

Trehalose-6-phosphate synthase is the key enzyme for biosynthesis of trehalose, the major soluble carbohydrate in resting cells of yeast. This enzyme was purified from a strain of Saccharomyces cerevisiae lacking vacuolar proteases. It was found to be a multimeric protein of 630 kDa. Monoclonal antibodies were raised against its smallest subunit (56 kDa) and used for screening a yeast cDNA library. This yielded an immunopositive cDNA clone of 1.7 kb, containing an open reading frame of 1485 base pairs. Its sequence, called TPS1 (for trehalose-6-phosphate synthase), was represented by a single gene in the yeast genome and was found to be almost identical with the recently sequenced CIF1, a gene important for carbon catabolite inactivation, believed to be allelic with FDP1. A mutant obtained by disruption of TPS1 had a very low activity of trehalose-6-phosphate synthase, indicating that TPS1 is an important component of the enzyme. The mutant also showed a growth defect when transferred from glycerol to glucose, a phenotype similar to that of the cif1 and fdp1 mutants deficient in carbon catabolite inactivation. Thus, the smallest subunit of the biosynthetic enzyme trehalose-6-phosphate synthase appears to have, in addition, a central regulatory role in the carbohydrate metabolism of yeast.

Abbreviations
TPS

trehalose-6-phosphate synthase

TPP

trehalose-6-phosphate phosphatase

YEP

yeast extract/bactopeptone

Enzymes
 

Trehalose-6-phosphate synthase (EC 2.4.1.15)

 

trehalose-6-phosphate phosphatase (EC 3.1.3.12)

 

neutral trehalase (EC 3.2.1.28)

REFERENCES

  1. Top of page
  2. Abstract
  3. REFERENCES
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