Note. Part of this report was presented at the XVth International Carbohydrate Symposium, Yokohama, Japan, August 12–17, 1990.
Structural investigation on the lipopolysaccharide of Escherichia coli rough mutant F653 representing the R3 core type
Article first published online: 3 MAR 2005
European Journal of Biochemistry
Volume 203, Issue 1-2, pages 127–134, January 1992
How to Cite
HAISHIMA, Y., HOLST, O. and BRADE, H. (1992), Structural investigation on the lipopolysaccharide of Escherichia coli rough mutant F653 representing the R3 core type. European Journal of Biochemistry, 203: 127–134. doi: 10.1111/j.1432-1033.1992.tb19837.x
- Issue published online: 3 MAR 2005
- Article first published online: 3 MAR 2005
- (Received June 4/September 18, 1991) – EJB 91 0723
The chemical structure of the core oligosaccharide of the lipopolysaccharide isolated from Escherichia coli rough mutant F653, representing the enterobacterial R3 core type, was investigated by quantitative and methylation analyses, nuclear magnetic resonance spectroscopy, gas-liquid chromatography/mass spectrometry, and determined as
All sugars are present as α-pyranosides but the anomeric configurations of the 3-deoxy-D-manno-octulopyranosonic acid (Kdo) residues could not be determined. The third Kdo and the heptose-linked GlcN residue are present in nonstoichiometric amounts; the GlcN residues may be, at least partially, N-acetylated.