Structural investigation on the lipopolysaccharide of Escherichia coli rough mutant F653 representing the R3 core type

Authors

  • Yuji HAISHIMA,

    1. Division of Biochemical Microbiology, Institut für Experimentelle Biologie und Medizin, Forschungsinstitut Borstel, Federal Republic of Germany
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  • Otto HOLST,

    1. Division of Biochemical Microbiology, Institut für Experimentelle Biologie und Medizin, Forschungsinstitut Borstel, Federal Republic of Germany
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  • Helmut BRADE

    Corresponding author
    1. Division of Biochemical Microbiology, Institut für Experimentelle Biologie und Medizin, Forschungsinstitut Borstel, Federal Republic of Germany
      Correspondence to H. Brade, Forschungsinstitut Borstel, Parkallee 22, W-2061 Borstel, Federal Republic of Germany
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  • Note. Part of this report was presented at the XVth International Carbohydrate Symposium, Yokohama, Japan, August 12–17, 1990.

Correspondence to H. Brade, Forschungsinstitut Borstel, Parkallee 22, W-2061 Borstel, Federal Republic of Germany

Abstract

The chemical structure of the core oligosaccharide of the lipopolysaccharide isolated from Escherichia coli rough mutant F653, representing the enterobacterial R3 core type, was investigated by quantitative and methylation analyses, nuclear magnetic resonance spectroscopy, gas-liquid chromatography/mass spectrometry, and determined as

image

All sugars are present as α-pyranosides but the anomeric configurations of the 3-deoxy-D-manno-octulopyranosonic acid (Kdo) residues could not be determined. The third Kdo and the heptose-linked GlcN residue are present in nonstoichiometric amounts; the GlcN residues may be, at least partially, N-acetylated.

Abbreviations
CI-MS

chemical ionisation mass spectrometry

COSY

correlation spectroscopy

EI-MS

electron impact mass spectrometry

Hep

l-glycero-d-manno-heptopyranose

Kdh

3-deoxyheptulopyranosonic acid

Kdo

3-deoxy-d-manno-octulopyranosonic acid

LD-MS

laser desorption mass spectrometry

LPS-HF

de-O-acylated and dephosphorylated lipopolysaccharide

LPS-OH

de-O-acylated lipopolysaccharide

OS-HF

dephosphorylated core oligosaccharide

Ancillary