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Abstract

  1. Top of page
  2. Abstract
  3. REFERENCES

Complete structures, including the location of N-acetylneuraminic acid (Neu5Ac) residues, were assigned for the core regions of Campylobacter jejuni serotypes O:1, O:4, and O:23 and O:36 lipopolysaccharides (LPS). In continuation of earlier studies, structure determinations of liberated oligosaccharides and, where necessary, of intact LPS, were by 1H-NMR spectroscopy, Smith degradation, chromium trioxide and enzymic degradations, in conjunction with methylation studies supported by fast-atom-bombardment mass spectrometry and linkage analyses by gas chromatography/mass spectrometry. It was concluded on the basis of the following structures, in which each was linked 1[RIGHTWARDS ARROW]5 to a terminal 3-deoxy-D-manno-octulosonic acid residue, that the core regions with qualititatively similar sugar compositions showed serotypic differences in one or more of their sequences, linkage types, and anomeric configurations:

  • image

The outer regions of each structure carry Neu5Ac residues linked 2[RIGHTWARDS ARROW]3 to available β-D-Galp residues and show striking similarities with various glycosphingolipids of the ganglioside family. However, Neu5Ac epitopes are not apparently involved in determining serospecificity.

Abbreviations
1D and 2D

one- and two-dimensional

COSY

correlation spectroscopy

COSYRCT

relayed coherence transfer correlation spectroscopy

FAB-MS

fast-atom-bombardment mass spectrometry

GC/MS

combined gas chromatography/mass spectrometry

GPC

gel permeation chromatography

LD-Hep

L-glycero-D-manno-heptose

Kdo

3-deoxy-D-manno-octulosonic acid

LPS

lipopolysaccharide

Neu5Ac

N-acetylneuraminic acid

OS

oligo-saccharide

PEtn

phosphorylethanolamine

Enzymes
 

β-N-Acetyglucosaminidase (EC 3.2.1.30)

 

β-galactosidase (EC 3.2.1.23)

 

sialidase (EC 3.2.1.18)

REFERENCES

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  2. Abstract
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