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Primary structure of the human muscle acetylcholine receptor

cDNA cloning of the γ and ɛ subunits

Authors


  • Note. The novel nucleotide sequence data published here has been deposited with the EMBL sequence data bank and is available under the accession number X66403.

Correspondence to D. Beeson, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, England OX3 9DU

Abstract

cDNA sequences encompassing the full coding region for the human muscle acetylcholine receptor (AChR) ɛ and γ subunits have been isolated. The deduced amino-acid sequences indicate that the mature ɛ subunit contains 473 amino acids and is preceded by a 20-amino-acid signal peptide. As predicted from genomic clones, the γ subunit contains 495 amino acids preceded by a 22-amino-acid signal peptide. In common with the human α, β, γ, and δ subunits the ɛ subunit is highly conserved between mammalian species. The ɛ subunit gene is not closely linked to the γ and δ subunits on chromosome 2 but rather is located with the β subunit on chromosome 17. Expression of the α-, β-, γ-, δ-, and ɛ-subunit cRNAs in rabbit-reticulocyte lysates followed by analysis on SDS/PAGE show glycosylated proteins with apparent molecular masses of 44–60 kDa.

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