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Abstract

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  2. Abstract
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Mouse and human cDNA encoding AES (amino-terminal enhancer of split) and ESG (enhancer of split groucho) proteins with strong similarity to Drosophila enhancer of split groucho protein were isolated and sequenced. Mouse AES-1 and AES-2 proteins, probably resulting from alternative splicing, contain 202 and 196 amino acids, respectively, while mouse ESG protein consists of 771 amino acids. The amino acid sequences of mouse and human AES proteins were found to exhibit approximately 50% identity to the amino-terminal region of Drosophila groucho, mouse ESG and human transducin-like enhancer of split (TLE) proteins. Mouse AES transcripts of 1.5 kb and 1.2 kb were abundantly expressed in muscle, heart and brain. Human AES transcripts of 1.6 kb and 1.4 kb were predominantly present in muscle, heart and placenta. Mouse ESG (homolog of human TLE 3) transcripts of 3.3 kb and 4.0 kb were found only in testis, while human TLE 1 transcripts of 4.5 kb was more abundant in muscle and placenta compared to heart, brain, lung, liver, kidney and pancreas. Human AES, TLE 1 and TLE 3 genes were mapped to chromosomes 19, 9 and 15, respectively, using human and Chinese hamster hybrid cell lines.

Abbreviations
AES

mammalian protein exhibiting strong similarity to amino-terminal region of Drosophila enhancer of split groucho protein

cdc2K

cdc2-kinase site

CKII

casein kinase II site

ESG

mammalian homolog of enhancer of split groucho protein

EST

expressed sequence tag

G-protein

guanine-nucleotide-binding regulatory protein

TLE

transducin-like enhancer of split

NLS

nuclear-localization sequence

WD-40 repeat

the repeat of approximately 40 amino acids demarcated by Trp-Asp (WD)

REFERENCES

  1. Top of page
  2. Abstract
  3. REFERENCES