Note. The permanent address of L. Acerenza is Seccion Biofisica, Facultad de Ciencias, Montevideo, Uruguay.
A universal method for achieving increases in metabolite production
Article first published online: 3 MAR 2005
European Journal of Biochemistry
Volume 216, Issue 2, pages 361–367, September 1993
How to Cite
KACSER, H. and ACERENZA, L. (1993), A universal method for achieving increases in metabolite production. European Journal of Biochemistry, 216: 361–367. doi: 10.1111/j.1432-1033.1993.tb18153.x
- Issue published online: 3 MAR 2005
- Article first published online: 3 MAR 2005
- (Received April 13, 1993) – EJB 93 0525/6
To increase the in vivo output of a chosen metabolite requires the increase of a number of enzyme concentrations. The enzymes are identified as those leading directly from output back to the input(s). This identification will reveal a number of branch points leading to other parts of metabolism which should not be perturbed. A simple calculation gives the enzyme multipliers, factors by which the identified enzyme concentrations should be increased to generate a given increase in the output flux. The net result of this procedure will be to extract any desired increase in flux while leaving the rest of metabolism to growth and other important functions of the cell unchanged. The method is entirely general.