Protein glycosylation

Structural and functional aspects

Authors

  • Halina LIS,

    1. Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, Israel
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  • Nathan SHARON

    Corresponding author
    1. Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, Israel
      Correspondence to N. Sharon, Department of Membrane Research and Biophysics, The Weizmann Institute of Science, IL-76100 Rehovot, Israel
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Correspondence to N. Sharon, Department of Membrane Research and Biophysics, The Weizmann Institute of Science, IL-76100 Rehovot, Israel

Abstract

During the last decade, there have been enormous advances in our knowledge of glycoproteins and the stage has been set for the biotechnological production of many of them for therapeutic use. These advances are reviewed, with special emphasis on the structure and function of the glycoproteins (excluding the proteoglycans). Current methods for structural analysis of glycoproteins are surveyed, as are novel carbohydrate–peptide linking groups, and mono- and oligo-saccharide constituents found in these macromolecules. The possible roles of the carbohydrate units in modulating the physicochemical and biological properties of the parent proteins are discussed, and evidence is presented on their roles as recognition determinants between molecules and cells, or cell and cells. Finally, examples are given of changes that occur in the carbohydrates of soluble and cell-surface glycoproteins during differentiation, growth and malignancy, which further highlight the important role of these substances in health and disease.

Abbreviations
CHO

Chinese hamster ovary

GlPtdIns

glycosyl-phosphatidylinositol

 

GM-CSF granulocyte-macrophage colony stimulating factor

hCG

human chorionic gonadotropin

LH

lutotropin

N-CAM

neural cell adhesion molecule

tPA

tissue plasminogen activator

Ancillary