SEARCH

SEARCH BY CITATION

Abstract

  1. Top of page
  2. Abstract
  3. References

The most relevant allergen of the storage mite Lepidoglyphus destructor (Lep d I) has been characterized. Lep d I is a monomer protein of 13273 Da. The primary structure of Lep d I was determined by N-terminal Edman degradation and partially confirmed by cDNA sequencing. Sequence polymorphism was observed at six positions, with non-conservative substitutions in three of them. No potential N-glycosylation site was revealed by peptide sequencing, The 125-residue sequence of Lep d I shows approximately 40% identity (including the six cysteines) with the overlapping regions of group II allergens from the genus Dermatophagoides, which, however, do not share common allergenic epitopes with Lep d I.

Abbreviations
RAST

radio allergosorbent test

3′-RACE

rapid amplification of cDNA 3′ end

Enzymes
 

Trypsin (EC 3.4.21.4)

 

chymotrypsin (EC 3.4.21.1)

 

endoproteinase Glu-C (EC 3.4.21.19)

 

endoproteinase Asp-N (EC 3.4.24.33)

 

proline-specific endopeptidase (EC 3.4.21.26)

References

  1. Top of page
  2. Abstract
  3. References
  • 1
    Voorhorst, R., Spieksma-Boezeman, M. I. A. & Spieksma, F. T. M. (1964) Is a mite (Dermatophagoides sp.) the producer of the house-dust allergen Allerg. Asthma 10, 329334.
  • 2
    Wraith, D. G., Cunnington, A. M. & Seymour, W. M. (1979) The role and allergenic importance of storage mites in house dust and other environments, Clin. Allergy 9, 545561.
  • 3
    Cuthbert, O. D., Brostoff, J., Wraith, D. G. & Brighton, W. D. (1979) ‘Barn allergy’: asthma and rhinitis due to storage mites, Clin. Allergy 9, 229236.
  • 4
    Van Hage-Hamsten, M., Johansson, S. G. O., Hoglund, S., Tull, P., Wiren, A. & Zetterstrom, O. (1985) Storage mite allergy is common in a farming population, Clin. Allergy 15, 555564.
  • 5
    Korsgaard, J., Dahl, R., Iversen, M. & Hallas, T. (1985) Storage mites as a cause of bronchial asthma in Denmark, Allergol. Immunopathol. 13, 143149.
  • 6
    Fernández-Caldas, E., Trudeau, W. L., García-Ramos, B. A. E., van Hage-Hamsten, M., Johansson, S. G. O. & Lockey, R. F. (1991) Sensitization to house dust and storage mites in urban dwellers, J. Allergy Clin. Immunol. 87, 322
  • 7
    Luczynska, C. M., Griffin, P., Davies, R. J. & Topping, M. D. (1990) Prevalence of specific IgE to storage mites (A. siro, L. destructor and T. longior) in an urban population and cross-reactivity with the house dust mite (D. pteronyssinus), Clin. Exp. Allergy 20, 403406.
  • 8
    Iversen, M. & Dahl, R. (1990) Allergy to storage mites in asthmatic patients and its relation to damp housing conditions, Allergy 45, 8185.
  • 9
    Johansson, E., Van Hage-Hamsten, M. & Johansson, S. G. O. (1988) Demonstration of allergen components in the storage mite Lepidoglyphus destructor by an immunoblotting technique, Int. Arch. Allergy Appl. Immunol. 85, 813.
  • 10
    Ventas, P., Carreira, J. & Polo, F. (1991) Identification of IgE-binding proteins from Lepidoglyphus destructor and production of monoclonal antibodies to a major allergen, Immunol. Lett. 29, 229234.
  • 11
    Ventas, P., Carreira, J. & Polo, F. (1992) Purification and characterization of Lep d I, a major allergen from the mite Lepidoglyphus destructor, Clin. Exp. Allergy 22, 454460.
  • 12
    Marsh, D. G., Goodfriend, L., King, T. P., Lowenstein, H. & Platts-Mills, T. (1986) Allergen nomenclature, Bull. W. H. O. 64, 767770.
  • 13
    Giménez-Gallego, G., Conn, G., Hatcher, V. B. & Thomas, K. A. (1986) The complete amino acid sequence of human brain-derived acidic fibroblast growth factor, Biochem. Biophys. Res. Commun. 138, 611617.
  • 14
    Marchuk, D., Drumm, M., Saulino, A. & Collins, F. S. (1991) Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products, Nucleic Acids Res. 19, 1154.
  • 15
    Sanger, F., Nicklen, S. & Coulson, A. R. (1977) DNA sequencing with chain-terminating inhibitors, Proc. Natl Acad. Sci. USA 74, 54635467.
  • 16
    Pearson, W. R. & Lipman, D. J. (1988) Improved tools for biological sequence comparison, Proc. Natl Acad. Sci. USA 85, 24442448.
  • 17
    Yuuki, T., Okumura, Y., Ando, T., Yamakawa, H., Suko, M., Haida, M. & Okudaira, H. (1991) Cloning and expression of cDNA coding for the major house dust mite allergen Der f II in Escherichia coli, Agric. Biol. Chem. 55, 12331238.
  • 18
    Griffith, I. J., Pollock, J., Klapper, D. G., Rogers, B. L. & Nault, A. K. (1991) Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed), Int. Arch. Allergy Appl. Immunol. 96, 296304.
  • 19
    Muthiah, R., Miller, M. & Kagen, S. (1991) Barn allergy: isolation and characterization of the major allergens of storage mites: L. destructor, J. Allergy Clin. Immunol. 87, 326.
  • 20
    Van Hage-Hamsten, M., Bergman, T., Johansson, E., Persson, B., Jörnvall, H., Harfast, B. & Johansson, S. G. O. (1992) N-terminal amino acid sequence of principal allergen of storage mite Lepidoglyphus destructor, Lancet 340, 614.
  • 21
    Nishiyama, C., Yuuki, T., Takai, T., Okumura, Y. & Okudaira, H. (1993) Determination of three disulfide bonds in a major house dust mite allergen, Der f II, Int. Arch. Allergy Immunol. 101, 159166.
  • 22
    Stewart, G. A., Bird, C. H. & Thompson, P. J. (1992) Do the group II dust mite allergens correspond to lysozyme J. Allergy Clin. Immunol. 90, 141142.
  • 23
    Johansson, E., Borga, A., Johansson, S. G. O. & van Hage-Hamsten, M. (1991) Immunoblot multi-allergen inhibition studies of allergenic cross-reactivity of the dust mites Lepidoglyphus destructor and Dermatophagoides pteronyssinus, Clin. Exp. Allergy 21, 511518.
  • 24
    Roebber, M., Klapper, D. G., Goodfriend, L., Bias, W. B., Hsu, S. H. & Marsh, D. G. (1985) Immunochemical and genetic studies of Amb. t. V (RaSG), an Ra5 homologue from giant ragweed pollen, J. Immunol. 134, 30623069.
  • 25
    Huang, S. K. & Marsh, D. G. (1991) Human T-cell responses to ragweed allergens: Amb V homologues, Immunology 73, 363365.
  • 26
    O'Hehir, R. E., Garman, R. G., Greestein, J. L. & Lamb, J. R. (1991) The specificity and regulation of T-cell responsiveness to allergens, Annu. Rev. Immunol. 9, 6795.
  • 27
    Berzofsky, J. A., Brett, S. J., Streicher, H. Z. & Takahashi, H. (1988) Antigen processing for presentation to T lymphocytes: Function, mechanisms, and implications for the T-cell repertoire, Immunol. Rev. 106, 531.
  • 28
    Rothbard, J. B. & Gefter, M. L. (1991) Interactions between peptides and MHC proteins, Annu. Rev. Immunol. 9, 527565.