H2: heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum

Composition and properties

Authors

  • Edgar SETZKE,

    1. Max-Planck-Institut für Terrestrische Mikrobiologie und Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität, Marburg, Germany
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  • Reiner HEDDERICH,

    1. Max-Planck-Institut für Terrestrische Mikrobiologie und Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität, Marburg, Germany
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  • Stefanie HEIDEN,

    1. Max-Planck-Institut für Terrestrische Mikrobiologie und Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität, Marburg, Germany
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  • Rudolf K. THAUER

    Corresponding author
    1. Max-Planck-Institut für Terrestrische Mikrobiologie und Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität, Marburg, Germany
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Correspondence to R. Thauer, Max-Planck-Institut für Terrestrische Mikrobiologie, Karl-von-Frisch-Straße, D-35043 Marburg/Lahn, Germany
Fax: +49 6421 285833.

Abstract

The reduction of the heterodisulfide (CoM-S-S-HTP) of coenzyme M (H-S-CoM) and N-7-mercaptoheptanoylthreonine phosphate (H-S-HTP) with H2 is an energy-conserving step in most methanogenic Archaea. In this study, we show that in Methanobacterium thermoautotrophicum (strain Marburg) this reaction is catalyzed by a stable H2: heterodisulfide oxidoreductase complex of F420-non-reducing hydrogenase and heterodisulfide reductase. This complex, which was loosely associated with the cytoplasmic membrane, was purified 17-fold with 80% yield to apparent homogeneity. The purified complex was composed of six different subunits of apparent molecular masses 80, 51, 41, 36, 21 and 17 kDa, and 1 mol complex, with apparent molecular mass 250 kDa, contained approximately 0.6 mol nickel, 0.9 mol FAD, 26 mol non-heme iron and 22 mol acid-labile sulfur. In 25 nM Chaps, the complex partially dissociated into two subcomplexes. The first subcomplex was was composed of the 51-, 41- and 17-kDa subunits; 1 mol trimer contained 0.7 mol nickel, 10 mol non-heme iron and 9 mol acid-labile sulfur and exhibited F420-non-reducing hydrogenase activity. The other subcomplex was composed of the 80-, 36- and 21-kDa subunits; 1 mol trimer contained 0.8 mol FAD, 22 mol non-heme iron and 15 mol acid-labile sulfur and exhibited heterodisulfide-reductase activity. The stimulatory effects of potassium phosphate, a membrane component, uracil derivatives and coenzyme F430 on the H2:heterodisulfide-oxidoreductase activity of the purified complex are described.

Abbreviation
H-S-CoM

coenzyme M or 2-mercaptoethanesulfonate

H-S-HTP

N-7-mercaptoheptanoylthreonine phosphate or N-7-mercaptoheptanoyl-O-phospho-l-threonine

CoM-S-S-HTP

heterodisulfide of H-S-CoM and H-S-HTP

d-CoM-S-S-HTP

heterodisulfie of H-S-CoM and N-7-mercaptoheptanoyl-O-phospho-d-threonine

dephospho CoM-S-S-HTP

heterodisulfide of H-S-CoM and N-7-mercaptoheptanoylthreonine

CoM-S-S-HxoTP

heterodisulfide of H-S-CoM and N-6-mercaptohexanoylthreonine phosphate

CoM-S-S-CoM

homodisulfide of H-S-CoM

HTP-S-S-HTP

homodisulfide of H-S-HTP

F420

coenzyme F420

Enzyme
 

H2:heterodisulfide oxidoreductase (EC 1.12.99.2)

 

heterodisulfide reductase (EC 1.97.1.–)

 

F420-non-reducing hydrogenase (EC 1.12.99.–)

 

F420-reducing hydrogenase (EC 1.12.99.1)

Ancillary