Note. The novel nucleotide sequence data published here have been submitted to the EMBL Data Library and are available under accession number X77214.
An inducible arylsulfatase of Volvox carteri with properties suitable for a reporter-gene system
Purification, characterization and molecular cloning
Article first published online: 3 MAR 2005
European Journal of Biochemistry
Volume 221, Issue 1, pages 143–150, April 1994
How to Cite
HALLMANN, A. and SUMPER, M. (1994), An inducible arylsulfatase of Volvox carteri with properties suitable for a reporter-gene system. European Journal of Biochemistry, 221: 143–150. doi: 10.1111/j.1432-1033.1994.tb18723.x
- Issue published online: 3 MAR 2005
- Article first published online: 3 MAR 2005
- (Received November 23, 1993/January 17, 1994) – EJB 93 1740/2
The multicellular green flagellate Volvox carteri synthesizes a periplasmic arylsulfatase in response to sulfur deprivation. The inducible enzyme has been purified to homogeneity and characterized. The corresponding gene and cDNA have been cloned. Determination of the sequence of genomic clones and comparisons to the cDNA sequence, revealed sixteen introns and seventeen exons that encode a 649-amino-acid polypeptide chain.
Since the arylsulfatase enzyme is readily assayed using chromogenic substrates, but is not detectable in cells grown in sulfate-containing medium, the gene encoding arylsulfatase may be useful as a reporter gene in V. carteri. In addition, the highly regulated promoter of the arylsufatase gene suggests its suitability as a tool for producing inducible expression vectors for cloned genes.