• 1
    Eigenbrodt, E. & Glossman, H. (1980) Glycolysis, one of the keys to cancer? Trends Pharmacol. Sci. 1, 240245.
  • 2
    Weinhouse, S. (1972) Glycolysis, respiration, and anomalous gene expression in experimental hepatomas: G. H. A. Clowes memorial lecture, Cancer Res. 32, 20072016.
  • 3
    Bustamante, E. & Pedersen, P. L. (1977) High aerobic glycolysis of rat hepatoma cells in culture: role of mitochondrial hexokinase, Proc. Natl Acad. Sci. USA 74, 37353739.
  • 4
    Schamhart, D. H. J., van de Poll, K. W. & van Wijt, R. (1979) Comparative studies of glucose metabolism in HTC, RLC, MH1C1, and Reuber H35 rat hepatoma cells, Cancer Res. 39, 10511055.
  • 5
    Nakashima, R. A., Paggi, M. G., Scott, L. J. & Pedersen, P. L. (1988) Purification and characterization of a bindable form of mitochondrial bound hexokinase from the highly glycolytic AS-30D rat hepatoma cell line, Cancer Res. 48, 913919.
  • 6
    Gelb, B. D., Adams, V., Jones, S. N., Griffin, L. D., MacGregor, G. R. & McCabe, E. R. B. (1992) Targeting of hexokinase 1 to liver and hepatoma mitochondria, Proc. Natl Acad. Sci. USA 89, 202206.
  • 7
    Rose, I. A. & Warms, J. V. B. (1967) Mitochondrial hexokinase: release, rebinding and location, J. Biol. Chem. 242, 16351645.
  • 8
    Kurokawa, M., Tokuoka, S., Oda, S., Tsubotani, E. & Ishibashi, S. (1981) Difference in efficiency of function between mitochondrial-bound hexokinase and non-bound one, Biochem. Int. 2, 645650.
  • 9
    Inui, M. & Ishibashi, S. (1979) Functioning of mitochondriabound hexokinase in rat brain in accordance with generation of ATP inside the organelle, J. Biochem. 85, 11511156.
  • 10
    Printz, R. L., Magnuson, M. A. & Granner, D. K. (1993) Mammalian glucokinase, Annu. Rev. Nutr. 13, 463496.
  • 11
    Iynedjian, P. B. (1993) Mammalian glucokinase and its gene, Biochem. J. 293, 113.
  • 12
    Loisseau, A. M., Rousseau, G. G. & Hue, L. (1985) Fructose 2,6-bisphosphate and the control of glycolysis by glucocorticoids and by other agents in rat hepatoma cells, Cancer Res. 45, 42634269.
  • 13
    Taketa, K., Shimamura, J., Ueda, M., Shimada, Y. & Kosaka, K. (1988) Profiles of carbohydrate-metabolizing enzymes in human hepatocellular carcinomas and preneoplastic livers, Cancer Res. 48, 467474.
  • 14
    Cifuentes, M. E., Espinet, C., Lange, A. J., Pilkis, S. J. & Hod, Y. (1991) Hormonal control of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene expression in rat hepatoma cells, J. Biol. Chem. 266, 15571563.
  • 15
    Darlington, G. J, Bernhard, H. P., Miller, R. A. & Ruddle, F. H. (1980) Expression of liver phenotypes in cultured mouse hepatoma cells, J. Natl. Cancer Inst. 64, 809819.
  • 16
    Katz, N., Immenschuh, S., Gerbracht, U., Eigenbrodt, E., Föllmann, W. & Petzinger, E. (1992) Hormone-sensitive carbohydrate metabolism in rat hepatocyte-hepatoma hybrid cells, Eur. J. Cell. Biol. 57, 117123.
  • 17
    Hammond, K. D. & Balinsky, D. (1978) Activities of key gluconeogenic enzymes and glycogen synthase in rat and human livers, hepatomas and hepatoma cell cultures, Cancer Res. 38, 13171322.
  • 18
    Newgard, C. B., Hirsch, L. J., Foster, D. W. & McGarry, J. D. (1983) Studies on the mechanism by which exogenous glucose is converted into liver glycogen in the rat: a direct or an indirect pathway? J. Biol. Chem. 258, 80468052.
  • 19
    Katz, J. & McGarry, J. D. (1984) The glucose paradox. Is glucose a substrate for liver metabolism? J. Clin. Invest. 74, 19011909.
  • 20
    Lang, C. H., Bagby, G. J., Blakesley, H. L., Johnson, J. L. & Spitzer, J. J. (1986) Plasma glucose concentration determines direct versus indirect liver glycogen synthesis, Am. J. Physiol. 251, E584E590.
  • 21
    McGarry, J. D., Kuwajima, M., Newgard, C. B., Foster, D. W. & Katz, J. (1987) From dietary glucose to liver glycogen: the full circle round, Annu. Rev. Nutr. 7, 5173.
  • 22
    Kurland, I. J. & Pilkis, S. J. (1989) Indirect versus direct routes of hepatic glycogen synthesis, FASEB J. 3, 22772281.
  • 23
    Huang, M. T. & Veech, R. (1988) Role of the direct and indirect pathways for glycogen synthesis in rat liver in the postprandial state, J. Clin. Invest. 81, 872878.
  • 24
    Killary, A. M., Lugo, T. G. & Fournier, R. E. K. (1984) Isolation of thymidine kinase-deficient rat hepatoma cells by selection with bromodeoxyuridine, Hoechst 33258, and visible light, Biochem. Genet. 22, 201213.
  • 25
    Killary, A. M. & Fournier, R. E. K. (1984) A genetic analysis of extinction: Trans-dominant loci regulate expression of liver-specific traits in hepatoma hybrid cells, Cell 38, 523534.
  • 26
    Short, J. M., Wynshaw-Boris, A., Short, H. P. & Hanson, R. W. (1986) Characterization of the P-enolpyruvate carboxykinase (GTP) promoter-regulatory region. I. Multiple hormone regulatory elements and the effects of enhancers, J. Biol. Chem. 261, 97219726.
  • 27
    Magnuson, M. A., Quin, P. G. & Granner, D. K. (1987) Multihormonal regulation of phosphoenolpyruvate carboxykinase-choramphenicol acetyltransferase fusion genes: Insulin's effects oppose those of cAMP and dexamethasone, J. Biol. Chem. 262, 1491714920.
  • 28
    Hatzoglou, M., Park, E. A., Wynshaw-Boris, A., Cheng Kaung, H. & Hanson, R. W. (1988) Hormonal regulation of chimeric genes containing the P-enolpyruvate carboxykinase promoter regulatory region in hepatoma cells infected by murine retroviruses, J. Biol. Chem. 263, 1779817808.
  • 29
    Bosch, F., Hatzoglou, M., Park, E. A. & Hanson, R. W. (1990) Vanadate inhibits expression of the gene for P-enolpyruvate carboxykinase (GTP) in rat hepatoma cells, J. Biol. Chem. 265, 1367713682.
  • 30
    Hatzoglou, M., Bosch, F., Park, E. A. & Hanson, R. W. (1991) Hormonal control of interacting promoters introduced into cells by retroviruses, J. Biol. Chem. 266, 84168425.
  • 31
    Miller, A. D. & Rosman, G. J. (1989) Improved retroviral vectors for gene transfer and expression, Biotechniques 7, 980990.
  • 32
    Mann, R., Mulligan, R. C. & Baltimore, D. (1983) Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus, Cell 33, 153159.
  • 33
    Iynedjian, P. B., Ucla, C. & Mach, B. (1987) Molecular cloning of glucokinase cDNA, J. Biol. Chem. 262, 60326038.
  • 34
    Yoo-Warren, H., Monahan, J. E., Short, J., Short, H., Bruzel, A., Wynshaw-Boris, A., Meisner, H. M., Samols, D. & Hanson, R. W. (1983) Isolation and characterization of the gene coding for cytosolic P-enolpyruvate carboxykinase (GTP) from the rat, Proc. Natl Acad. Sci. USA 80, 36563660.
  • 35
    Beale, E. G., Chrapkiewicz, N. B., Scoble, H. A., Metz, R. J., Quick, D. P., Noble, R. L., Donelson, J. E., Biemann, K. & Granner, D. K. (1985) Rat hepatic cytosolic P-enolpyruvate carboxykinase (GTP). Structure of the protein, messenger RNA, and gene, J. Biol. Chem. 260, 1074810760.
  • 36
    Chirgwin, J. M., Przybyla, A. W., McDonald, R. J. & Rutter, W. J. (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease, Biochemistry 18, 52945299.
  • 37
    Davidson, A. L. & Arion, W. J. (1987) Factors underlying significant underestimations of glucokinase activity in crude liver extracts: physiological implications of higher cellular activity, Arch. Biochem. Biophys. 253, 156167.
  • 38
    Keppler, D. & Decker, K. (1981) Glycogen, in Methods of enzymatic analysis, (Bergmeyer, H. U., ed.) vol. 6, pp. 1118, Verlag Chemie GmbH, Weinheim, Germany .
  • 39
    Michal, G. (1981) Glucose 6-Phosphate, in Methods of enzymatic analysis, (Bergmeyer, H. U., ed. vol. 6, pp. 185190, Verlag Chemie GmbH, Weinheim, Germany .
  • 40
    Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (1989) Current protocols in molecular biology, John Wiley & Sons, New York .
  • 41
    Van Schaftingen, E., Lederer, B., Bartrons, R. & Hers, H.-G. (1982) A Kinetic Study of Pyrophosphate: Fructose-6-phosphotransferase from potato tubers. Application to a microassay of fructose 2,6-bisphosphate, Eur. J. Biochem. 129, 191195.
  • 42
    Miller, T. B. & Larner, J. (1973) Mechanism of control of hepatic glycogenesis by insulin, J. Biol. Chem. 248, 34833488.
  • 43
    Larner, J. (1990) Insulin and the stimulation of glycogen synthesis. The road from glycogen structure to glycogen synthase to cyclic AMP-dependent protein kinase to insulin mediators, Adv. Enzymol. Relat. Areas Mol. Biol. 63, 173231.
  • 44
    Roach, P. J. (1990) Control of glycogen synthase by hierarchal protein phosphorylation, FASEB J. 4, 29612968.
  • 45
    Claus, T. H., El-Maghrabi, M. R., Regen, D. M., Stewart, H. B., McGrane, M., Kountz, P. D., Nyfeler, F., Pilkis, J. & Pilkis, S. J. (1984) The role of fructose 2,6-bisphosphate in the regulation of carbohydrate metabolism, Curr. Topics Cell. Reg. 23, 5186.
  • 46
    Hue, L. & Rider, M. H. (1987) Role of fructose 2,6-bisphosphate in the control of glycolysis in mammalian tissues, Biochem. J. 245, 313324.
  • 47
    Hatzoglou, M., Lamers, W., Bosch, F., Winshaw-Boris, A., Clap, D. W. & Hanson, R. W. (1990) Hepatic gene transfer in animals using retroviruses containing the promoter from the gene for phosphoenolpyruvate carboxikinase, J. Biol. Chem. 265, 1728517293.
  • 48
    Pessin, J. E. & Bell, G. I. (1992) Mammalian facilitative glucose transporter family: structure and molecular regulation, Annu. Rev. Physiol. 54, 911930.
  • 49
    Bell., G. I., Burant, C. F., Takeda, J. & Gould, G. W. (1993) Structure and function of mammalian facilitative sugar transporters, J. Biol. Chem. 268, 1916119164.
  • 50
    Tal., M., Liang, Y., Najafi, H., Lodish, H. F. & Matschinsky, F. M. (1992) Expression and function of GLUT-1 and GLUT-2 glucose transporter isoforms in cells of cultured rat pancreatic islets, J. Biol. Chem. 267, 1724117247.
  • 51
    Giacari, A., Rossetti, L. (1992) Predominant role of gluconeogenesis in the hepatic glycogen repletion of diabetic rats, J. Clin. Invest. 89, 3645.
  • 52
    Meienhofer, M.-C., De Medicis, E., Cognet, M. & Kahn, A. (1987) Regulation of genes for glycolytic enzymes in cultured rat hepatoma cell lines, Eur. J. Biochem. 169, 237243.
  • 53
    Bergot, M.-O., Diaz-Guerra, M.-J. M., Puzenat, N., Raymond-jean, M. & Kahn, A. (1992) Cis-regulation of the L-type pyruvate kinase gene promoter by glucose, insulin and cyclic AMP, Nucleic Acid Res. 20, 18711878.
  • 54
    Granner, D. K. & Pilkis, S. (1990) The genes of hepatic glucose metabolism, J. Biol. Chem. 265, 1017310176.