Dedication. Dedicated to Prof. H. G. Floss on the occassion of his 60th birthday.
The (2R)-hydroxycarboxylate-viologen-oxidoreductase from Proteus vulgaris is a molybdenum-containing iron-sulphur protein
Article first published online: 3 MAR 2005
European Journal of Biochemistry
Volume 222, Issue 3, pages 1025–1032, June 1994
How to Cite
TRAUTWEIN, T., KRAUSS, F., LOTTSPEICH, F. and SIMON, H. (1994), The (2R)-hydroxycarboxylate-viologen-oxidoreductase from Proteus vulgaris is a molybdenum-containing iron-sulphur protein. European Journal of Biochemistry, 222: 1025–1032. doi: 10.1111/j.1432-1033.1994.tb18954.x
- Issue published online: 3 MAR 2005
- Article first published online: 3 MAR 2005
- (Received February 28, 1994) – EJB 94 0279/4
An oxidoreductase with an extremely broad substrate specificity reducing reversibly 2-oxocar-boxylates at the expense of reduced artificial redox mediators to (2R)-hydroxycarboxylates has been purified to a specific activity of up to 1800 μmol · min−1· mg−1 for the reduction of phenylpyruvate. The membrane-bound non-pyridine nucleotide-dependent enzyme appears in the form of various oligomers of the 80-kDa monomer.
The isoelectric point is 5.1. Based on a molecular mass of 80 kDa the enzyme contains up to one molybdenum, four iron and four sulphur atoms. After growth on 99Mo-labelled molybdate, enzyme and radioactivity coincided as shown by gel electrophoresis.
Permanganate oxidation delivers 0.7 mol pterin-6-carboxylic acid. The molybdenum cofactor is a mononucleotide. The enzyme is inhibited by cyanide. The first 20 amino acids have been determined. The enzyme belongs to the rare group of molybdoenzymes which possess no further prosthetic groups than the iron-sulphur clusters.