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Abstract

  1. Top of page
  2. Abstract
  3. REFERENCES

In the yeast Saccharomyces cerevisiae, accumulation of the non-reducing disaccharide trehalose is triggered by various stimuli that activate the heat-schock response. Several studies have shown a close correlation between trehalose levels and tolerance to heat stress, suggesting that trehalose may be a protectant which contributes to thermotolerance. In this study, we have examined mutants defective in genes coding for key enzymes involved in trehalose metabolism with respect to the heat-induced and stationary-phase-induced accumulation of trehalose and the acquisition of thermotolerance. Inactivation of either TPS1 or TPS2, encoding subunits of the trehalose-6-phosphate synthase/phosphatase complex, caused an inability to accumulate trehalose upon a mild heat-shock or upon initiation of the stationary phase and significantly reduced the levels of heat-induced and stationary-phase-induced thermotolerance. Deletion of NTH1, the gene coding for the neutral trehalase, resulted in a defect in trehalose mobilization during recovery from a heat shock which was paralleled by an abnormally slow decrease of thermotolerance. Our results provide strong genetic evidence that heat-induced synthesis of trehalose is an important factor for thermotolerance induction. In an accompanying study [Hottiger, T., De Virgilio, C., Hall, M. N., Boller, T. & Wiemken, A. (1993) Eur. J. Biochem. 219, 187–193], we present evidence that the function of heat-induced trehalose accumulation may be to increase the thermal stability of proteins.

Abbreviations
Tre6P

trehalose-6-phosphate

hsp

heat-shock protein(s)

TPS1

56-kDa subunit of trehalose-6-phosphate synthase/phosphatase complex

TPS2

102-kDa subunit of trehalose-6-phosphate synthase/phosphatase complex

TSL1

123-kDa subunit of trehalose-6-phosphate synthase/phosphatase complex

TPS3

homolog of TSL1

NTH1

neutral trehalase

Enzymes
 

Trehalose-6-P synthase [UDP-glucose: D-glucose-6-phosphate-1-glucosyltransferase] (EC 2.4.1.15)

 

trehalose-6-P phosphatase [trehalose-6-phosphate phosphohydrolase] (EC 3.1.3.12)

 

neutral trehalase (EC 3.2.1.28)

REFERENCES

  1. Top of page
  2. Abstract
  3. REFERENCES
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