Enzymically Produced Cyclic α-1,3-Linked and α-1,6-Linked Oligosaccharides of d-Glucose

Authors

  • Gregory L. Côté,

    Corresponding author
    1. Biopolymer Research Unit, National Center for Agricultural Research, Agricultural Research Service, United States Department of Agriculture, Illinois, USA
      Correspondence to G. L. Côté, ARS-USDA, NCAUR, 1815 N. University St., Peoria IL, 61604, USA
      Fax: 1 309 681 6689.
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  • Peter Biely

    1. Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia
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Correspondence to G. L. Côté, ARS-USDA, NCAUR, 1815 N. University St., Peoria IL, 61604, USA
Fax: 1 309 681 6689.

Abstract

A new type of bacterial enzyme hydrolyzed alternan (Leuconostoc mesenteroides NRRL B-1355 fraction S dextran, an alternating α-1,3–α-1,6-d-glucan) to give rise to a series of oligosaccharides. The oligosaccharide formed in the greatest proportion was a cyclic tetrasaccharide of d-glucosyl residues linked in an alternating α-1,3–α-1,6 fashion. Other saccharide products included isomaltose and α-d-glucopyranosyl-1,3–α-d-glucopyranosyl-1,6-d-glucose. Oligosaccharides of higher degrees of polymerization were also formed, and included α-d-glucosylated derivatives of the cyclic tetrasaccharide. This is the first report of a naturally produced cyclic tetrasaccharide.

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