The novel nucleotide sequence published here has been deposited with the EMBO data base and is available under accession number Z49993 and Z49994.
Molecular Cloning and Functional Expression of the Gene Encoding the Human Proteinase-Activated Receptor 2
Article first published online: 3 MAR 2005
European Journal of Biochemistry
Volume 232, Issue 1, pages 84–89, August 1995
How to Cite
Nystedt, S., Emilsson, K., Larsson, A.-K., Strömbeck, B. and Sundelin, J. (1995), Molecular Cloning and Functional Expression of the Gene Encoding the Human Proteinase-Activated Receptor 2. European Journal of Biochemistry, 232: 84–89. doi: 10.1111/j.1432-1033.1995.tb20784.x
- Issue published online: 3 MAR 2005
- Article first published online: 3 MAR 2005
- (Received 6 June 1995) – EJB 95 0692/2
- chromosomal mapping;
- guanosione-nucleotide-binding-protein-coupled receptor;
- molecular cloning;
We previously reported the molecular cloning of a mouse guanosine-nucleotide-binding-protein-coupled receptor similar to the thrombin receptor. Since the physiological agonist was unknown, the receptor was named proteinase-activated receptor 2. We describe here the cloning and functional expression of the gene encoding the corresponding human receptor. The gene is divided into two exons separated by about 14 kb intronic DNA. The deduced protein sequence is 397 amino acids long and 83% identical to the mouse receptor sequence. Within the extracellular amino terminus, the residues predicted to form the tethered agonist ligand differ between the two receptors; of the first six residues only four are conserved. At positions five and six, a lysine residue and a valine residue, respectively, have replaced arginine and leucine residues found in the mouse sequence. When the human receptor is expressed in Chinese hamster ovary cells, it can be activated by low nanomolar concentrations of the serine proteinase trypsin and by peptides made from the receptor sequence. Northern-blot analysis of receptor expression showed that the receptor transcript is widely expressed in human tissues with especially high levels in pancreas, liver, kidney, small intestine and colon. Moderate expression was detected in many organs but none in brain or skeletal muscle. By fluorescence in situ hybridization, the human proteinase-activated receptor 2 gene was mapped to chromosomal region 5q13, where, previously, the related thrombin receptor gene has been located.