Glutamine Increases Argininosuccinate Synthetase mRNA Levels in Rat'Hepatocytes

The Involvement of Cell Swelling

Authors

  • Muriel Quillard,

    1. Groupe de Biochimie et de Physiopathologie Digestive et Nutritionnelle et Institut Fédératif de Recherches Multidisciplinaires sur les Peptides no. 23, Faculté Médecine-Pharmacie de Rouen, France
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  • Annie Husson,

    1. Groupe de Biochimie et de Physiopathologie Digestive et Nutritionnelle et Institut Fédératif de Recherches Multidisciplinaires sur les Peptides no. 23, Faculté Médecine-Pharmacie de Rouen, France
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  • Alain Lavoinne

    Corresponding author
    1. Groupe de Biochimie et de Physiopathologie Digestive et Nutritionnelle et Institut Fédératif de Recherches Multidisciplinaires sur les Peptides no. 23, Faculté Médecine-Pharmacie de Rouen, France
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A. Lavoinne, Groupe de Biochimie et de Physiopathologie Digestive et Nutritionnelle, Faculté de Médecine-Pharmacie de Rouen, F-76803 St-Etienne-du-Rouvray Cédex, France
Fax:+33 35 66 44 50.

Abstract

Glutamine, one of the most efficient substrates in the urea cycle, was found to induce the accumulation of argininosuccinate synthetase (ASS) mRNA in primary cultured rat hepatocytes. The inducing action of glutamine was obtained at various stages of development and a half-maximal effect was observed at about 3 mM glutamine. This effect was apparent from 6 h and persisted for at least 48 h. NH4Cl addition up to 5 mM did not significantly change the ASS mRNA level. Like glutamine, hypoosmotic medium and aminoisobutyric acid (conditions known to increase cell volume) also increased the ASS mRNA level, which was, in contrast, decreased by hyperosmotic medium. These results demonstrate that the glutamine-induced swelling may participate in the observed increase of the ASS mRNA level. This increase in the ASS mRNA level was associated with an increase in ASS activity.

Abbreviations
ASS

argininosuccinate synthetase

ASL

Argininosuccinate lyase

PEPCK

phosphoenolpyruvate carboxykinase

Enzymes
 

Argininosuccinate synthetase (EC 6.3.4.5)

 

argininosuccinate lyase (EC 4.3.2.1)

 

phosphoenolpyruvate carboxykinase (EC 4.1.1.32)

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