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Keywords:

  • retinoic acid;
  • retinaldehyde;
  • aldehyde dehydrogenase;
  • retinaldehyde dehydrogenase;
  • P19 teratocarcinoma cells

Abstract

  1. Top of page
  2. Abstract
  3. References

Retinoic acid, a developmental signal implicated in the formation of the neural axis, is present at high levels in the early embryonic trunk region, where it is synthesized by a novel dehydrogenase. Here we show that the same enzyme is inducible by retinoic acid in P19 teratocarcinoma cells, and we report the cloning from P19 cells of a cDNA encoding a novel dehydrogenase, named retinaldehyde dehydrogenase-2 (RALDH-2). Expression in COS cells shows RALDH-2 to be highly effective in oxidation of retinaldehyde, with no detectable activity on any other aldehyde tested. In situ hybridization histochemistry on the embryonic trunk reveales RALDH-2 mRNA both in mesoderm and neuroectoderm, with highest neuroectodermal expression in the ventral horn of the spinal cord at two restricted locations along the anteroposterior axis, presumably the subpopulation of motoneurons that innervate the limbs.

Abbreviations
AHD2

murine class 1 aldehyde dehydrogenase

CRABP

cellular retinoic-acid binding protein

DMEM

Dulbecco's modified Eagle's medium

E10

embryonic day 10, with day of conception defined as E0

P19 cells

a murine teratocarcinojna cell line

RALDH

retinaldehyde dehydrogenase

RPE

retinal pigment epithelium

RT

reverse transcription

Enzymes
 

Aldehyde dehydrogenase (EC 4.2.1.3)

References

  1. Top of page
  2. Abstract
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