On the Mechanism of Selenium Tolerance in Selenium-Accumulating Plants

Purification and Characterization of a Specific Selenocysteine Methyltransferase from Cultured Cells of Astragalus Bisculatus

Authors


A. Böck, Institut für Genetik und Mikrobiologie, Maria-Ward-Str. 1a, D-80638 Müchen, Germany

Abstract

Selected members of the genus Astragulus (Fabaceae) are known for their ability to accumulate high levels of selenium, mainly in the form of Se-methyl-selenocysteine. With the aid of cell cultures we have investigated the molecular basis for selenium tolerance of these plants. It is shown that cultured cells from a selenium-accumulating Astragalus species synthesize Se-methyl-selenocysteine in contrast to those of a non-accumulating species and do not unspecifically incorporate selenium into proteins. The purification and biochemical characterization of a selenocysteine methyltransferase from cultured Astragalus bisculatus cells is described, which does not accept cysteine as a substrate. We propose that this enzyme plays a crucial role in conferring selenium tolerance.

Abbreviations
AdoMet

S-adenosylmethionine

SecMT

selenocysteine methyltransferase

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