Note. The novel nucleotide sequence data published here have been submitted to the EMBL sequence data bank and are available under accession number X96735.
The Human Gene FALL39 and Processing of the Cathelin Precursor to the Antibacterial Peptide LL-37 in Granulocytes
Article first published online: 31 AUG 2004
European Journal of Biochemistry
Volume 238, Issue 2, pages 325–332, June 1996
How to Cite
Gudmundsson, G. H., Agerberth, B., Odeberg, J., Bergman, T., Olsson, B. and Salcedo, R. (1996), The Human Gene FALL39 and Processing of the Cathelin Precursor to the Antibacterial Peptide LL-37 in Granulocytes. European Journal of Biochemistry, 238: 325–332. doi: 10.1111/j.1432-1033.1996.0325z.x
- Issue published online: 31 AUG 2004
- Article first published online: 31 AUG 2004
- (Received 19 December 1995) – EJB 95 2080/2
- dipeptidyl-peptidase I;
- flow cytometry;
- genomic cloning;
- peptide antibiotic;
- precursor processing
The peptide FA-LL-37, previously termed FALL-39, was originally predicted from an ORF of a cDNA clone isolated from a human bone marrow library. This peptide was synthesized and found to have antibacterial activity. We have now characterized and sequenced the complete gene for FA-LL-37, termed FALL39. It is a compact gene of 1963 bp with four exons. Exons 1–3 code for a signal sequence and the cathelin region. Exon 4 contains the information for the mature antibacterial peptide. Our results indicate that FALL39 is the only member of the cathelin gene family present in the human genome. Potential binding sites for acute-phase-response factors are identified in the promoter and in intron 2. A possible role for the cytokine interleukin-6 in the regulation of FALL39 is discussed. Anti-(FA-LL-37) IgG located the peptide in granulocytes and we isolated the mature peptide from these cells after degranulation. Structural analysis determined the mature peptide to be LL-37. To obtain LL-37 for antibacterial assays, synthetic FA-LL-37 was degraded with dipeptidyl-peptidase I. This analysis showed that mature LL-37 is a potent antibacterial peptide.