The primary structure of HSP-7, a 14-kDa protein isolated from stallion seminal plasma, has been determined. HSP-7 belongs to the spermadhesin protein family, shares 98% sequence identity with the boar seminal plasma protein AWN, and, like its boar homolog, displays zona-pellucida-binding activity. Despite these conserved structural and functional features, the equine and porcine spermadhesins differ in their topography on spermatozoa.
horse seminal plasma
matrix-assisted laser-desorption ionization time-of-flight
boar seminal plasma protein AWN
acidic seminal fluid protein
Trypsin (EC 184.108.40.206)
α-chymotrypsin (EC 3.4.21.I)
endoproteinase Lys-C (EC 220.127.116.11)