Note. The novel nucleotide sequence presented here has been submitted to the GenBank data bank and is available under accession number D38585.
Molecular Cloning and Characterization of a Transcription Factor for the C-Type Natriuretic Peptide Gene Promoter
Article first published online: 23 JUL 2004
European Journal of Biochemistry
Volume 242, Issue 3, pages 460–466, December 1996
How to Cite
Ohta, S., Shimekake, Y. and Nagata, K. (1996), Molecular Cloning and Characterization of a Transcription Factor for the C-Type Natriuretic Peptide Gene Promoter. European Journal of Biochemistry, 242: 460–466. doi: 10.1111/j.1432-1033.1996.460rr.x
- Issue published online: 23 JUL 2004
- Article first published online: 23 JUL 2004
- (Received 24 June/19 September 1996) – EJB 96 0933/1
- C-type natriuretic peptide;
- transcription factor;
- transforming growth factor-β
Our previous studies on the promoter function of the human C-type natriuretic peptide (CNP) gene revealed the existence of two GC-rich cis elements essential for gene transcription in rat pituitary-derived GH3 cells. To isolate transcription factors that bind to those GC-rich elements, we screened a λZAP cDNA library derived from GH3 cells by Southwestern screening. Several positive clones with specific binding abilities were obtained, and one was identical as TSC-22, a speculated transcriptional modulator stimulated by transforming growth factor β (TGF-β) of unknown function. TSC-22 significantly enhanced CNP promoter activity in GH3 cells. We further cloned a 1.8-kb full-length human TSC-22 cDNA from a fetal kidney cDNA library by a combination of polymerase chain reaction and the rapid amplification of the cDNA ends technique. In adults, human TSC-22 mRNA was highly expressed in brain, lung and heart. TSC-22 gene expression in GH3 and human aortic endothelial cells was stimulated by cytokines including TGF-β in correlation with the CNP mRNA increase. These results suggest that TSC-22 is a transcriptional regulator of the CNP gene and transmits signals from cytokines, such as TGF-β, to CNP gene expression.