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Keywords:

  • silver protein;
  • pmel 17;
  • melanogenesis;
  • 5,6-dihydroxyindole-2-carboxylic acid polymerization

Abstract

  1. Top of page
  2. Abstract
  3. References

Recent advances in melanogenesis have focused on the role of dihydroxyindole-2-carboxylic acid [(HO)2IndCOOH]. For example, it has been shown that formation of (HO)2IndCOOH from dopachrome is catalyzed by dopachrome tautomerase, that the melanogenic protein tyrosinase-related protein (TRP)-1 can oxidize (HO)2IndCOOH to its indole quinone, that (HO)2IndCOOH-melanms can be synthesized chemically, that mammalian melanins are naturally rich in (HO)2IndCOOH subunits, and that (HO)2IndCOOH is incorporated into melanins of melanomas in mice. The question thus emerges as to the mechanism(s) by which (HO)2IndCOOH and other precursors become incorporated into melanins in vivo. Accordingly, an activity was partially purified that catalyzed melanin formation with (HO)2IndCOOH as a substrate. Analyses of the (HO)2IndCOOH polymerization factor from Cloudman melanoma cells revealed the following: it was proteinaceous in that it was heat labile and destroyed by proteinase K; it was a glycoprotein in that it adhered to wheat germ agglutinin and was eluted with N-acetyl glucosamine; it was located predominantly in the melanosomal fraction of cell homogenates; the activity was reduced by exposure to the metal chelators EDTA and EGTA, but not by phenylthiourea, a tyrosinase inhibitor; the (HO)2IndCOOH polymerization reaction was inhibited by superoxide dismutase. In addition, the activity was found with the mouse pmel 17/silver locus protein immunopurified from human melanoma cells, and was significantly reduced in extracts of mouse melanocytes cultured from silver (si/si) mice compared to extracts from Si/Si melanocytes. In summary, an activity has been identified in human and mouse melanoma cells that catalyzes the superoxide-dependent polymerization of (HO)2IndCOOH to melanin in vitro, and appears to be a function of the pmel 17/silver protein of the human pmel 17 gene and the mouse silver locus.

Abbreviations
(HO)2IndCOOH

5,6-dihydroxyindole-2-carboxylic acid

TRP

tyrosinase-related protein

HGPRT-1

hypoxanthine guanine phosphoribosyl transferase negative

PhMeSO2F

phenylmethylsulfonylfluoride

SOD

superoxide dismutase

GST

glutathione S-transferase

References

  1. Top of page
  2. Abstract
  3. References
  • Adema, G. J., deBoer, A. J., Vogel, A. M., Loenen, A. M. & Figdor, C. G. (1994) Molecular characterization of the melanocyte lineage-specific antigen gp100, J. Biol. Chem. 269, 2012620133.
  • Aroca, P., Solano, F., Salinas, C., Garcia-Borron, J. C. & Lozano, J. A. (1992) Regulation of the final phase of mammalian melanogenesis, Eur. J. Biochem. 208, 155163.
  • Barber, J. I., Townsend, D., Olds, D. P. & King, R. A. (1984) Dopachrome oxidoreductase: a new enzyme in the pigment pathway, J. Invest. Dermatol. 83, 145149.
  • Cadenas, E. (1989) Biochemistry of oxygen toxicity, Annu. Rev. Biochem. 58, 79110.
  • Chakraborty, A. K., Mishima, Y., Inazu, M., Hatta, S. & Ichihashi, M. (1989) Melanogenic regulatory factors in coated vesicles from melanoma cells, J. Invest. Dermatol. 93, 616620.
  • Chakraborty, A. K., Kwon, B. S., Bennett, D. C. & Pawelek, J. M. (1994) Identification of a potential late step in melanogenesis: enzymatic conversion of DHICA to melanin, J. Invest. Dermatol. 102, 552.
  • Chakraborty, A. K., Osber, M. P. & Pawelek, J. M. (1993) Conversion of dihydroxyindole-2-carboxylic acid to melanin, Pigment Cell Res. 6, 280.
  • Donatien, P. D. & Orlow, S. J. (1995) Interaction of melanosomal proteins with melanin, Eur. J. Biochem. 232, 157164.
  • Dunn, L. C. & Thigpen, L. W. (1930) The silver mouse, a recessive color variation, J. Hered. 21, 495498.
  • Gown, A. M., Vogel, A. M., Hoak, M., Gough, F. & McNutt, M. A. (1986) Monoclonal antibodies specific for melanocyte tumors distinguish subpopulations of melanocytes, Am. J. Pathol. 123, 195203.
  • Ito, S. (1986) Reexamination of the structure of eumelanin, Biochim. Biophys. Acta. 883, 155161.
  • Ito, S. & Nicol, J. A. C. (1974) Isolation of oligomers of 5,6-dihydroxy-indole-2-carboxylic acid from the eye of the catfish, Biochem. J. 143, 207217.
  • Kawakami, Y., Eliyahu, S., Delgado, D. H., Robbins, P. F., Sakaguchi, K., Appella, E., Yannelli, J. R., Adema, G. J., Miki, T. & Rosenberg, S. A. (1994) Identification of a human melanoma antigen recognized by tumor-infiltrating lymphocytes associated with in vivo tumor rejection. Proc. Natl. Acad. Sci. USA 91, 64586462.
  • Kobayashi, T., Urabe, K., Winder, A., Tsukamoto, K., Brewington, T., Imokawa, G., Potterf, B. & Hearing, V. J. (1994a) DHICA oxidase activity of TRP1 and Interactions with Other Melanogenic enzymes, Pigm. Cell Res. 7, 227234.
  • Kobayashi, T., Urabe, K., Winder, A., Jimenez-Cervantes, C., Imokawa, G., Brewington, T., Solano, F., Garcia-Borron, J. C. & Hearing, V. J. (1994b) Tyrosinase related protein 1 (TRP1) functions as a DHICA oxidase in melanin biosynthesis, EMBO J. 13, 58185825.
  • Kobayashi, T., Urabe, K., Orlow, S. J., Higashi, K., Imokawa, G., Kwon, B. S., Potterf, B. & Hearing, V. J. (1994c) The Pmell7/silver locus protein: Characterization and investigation of its melanogenic function, J. Biol. Chem. 269, 2919829205.
  • Korner, A. & Pawelek, J. (1980) Dopachrome conversion: A possible regulatory point in melanin biosynthesis, J. Invest. Dermatol. 75, 192195.
  • Kwon, B. S., Halaban, R., Kim, G. S., Usack, L., Pomerantz, S. & Haq, A. K. (1987) A melanocyte-specific complementary DNA clone whose expression is inducible by melanotropin and isobutylmethyl xanthine, Mol. Biol. Med. 4, 339355.
  • Kwon, B. S., Chintamaneni, C., Kozak, C. A., Copeland, B. G., Gilbert, D. J., Jenkins, N., Barton, D., Francke, J. U., Kobayashi, Y. & Kim, K. K. (1991) A melanocyte-specific gene, pmel 17, maps near the silver coat color locus on mouse chromosome 10 and is in a syntenic region on human chromosome 12, Proc. Natl Acad. Sci. USA 88, 92289232.
  • Kwon, B. S. (1993) Pigmentation genes: the tyrosinase gene family and the pmel 17 gene family, J. Invest. Dermatol. 100, 134S140S.
  • Kwon, B. S., Kim, K. K., Halaban, R. & Pickard, R. T. (1994) Characterization of mouse pmel 17 gene and silver locus, Pigm. Cell Res. 7, 394397.
  • Kwon, B. S., Halaban, R., Ponnazhagan, S., Kim, K., Chintamameni, C., Bennett, D. & Pickard, R. T. (1995) Mouse silver mutation is caused by a single base insertion in the putative cytoplasmic domain of pmel 17, Nucleic Acids Res. 23, 154158.
  • Marklund, S. & Marklund, G. (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase, Eur. J. Biochem. 47, 469474.
  • Misra, H. P. & Fridovich, I. (1972) The role of superoxide anion in the autooxidation of ephinephrine and a simple assay for superoxide dismutase, J. Biol. Chem. 247, 31703175.
  • Mochii, M., Agata, K. & Eguchi, G. (1991) Complete sequence and expression of a cDNA encoding a chicken 115-kDa melanosomal matrix protein, Pigm. Cell Res. 4, 4147.
  • Orlow, S. J., Osber, M. P. & Pawelek, J. M. (1992) Synthesis and characterization of melanins from dihydroxyindole-2-carboxylic acid and dihydroxyindole, Pigm. Cell Res. 5, 113121.
  • Pavel, S. (1994) Dopachrome tautomerase is not essential for DHICA formation, Pigm. Cell Res. 7, 123127.
  • Pawelek, J. M. (1991) After dopachrome Pigm. Cell Res. 4, 5362.
  • Pawelek, J., Korner, A., Bergstrom, A. & Bolognia, J. (1980) New regulators of melanin biosynthesis and the autodestruction of melanoma cells, Nature 286, 617619.
  • Pawelek, J. M. (1979) Evidence suggesting that a cyclic AMP-dependent protein kinase is a positive regulator of proliferation in Cloudman S91 melanoma cells, J. Cell. Physiol. 98, 619625.
  • Pawelek, J., Chakraborty, A. K., Osber, M. P., Orlow, S. J., Min, K. K., Rosenzweig, K. E. & Bolognia, J. L. (1992) Molecular cascades in UV-induced melanogenesis: A central role for melanotropins Pigm. Cell Res. 5, 348356.
  • Pawelek, J. M., Platt, J., Pugliese, P. T. & Chakraborty, A. K. (1994) Enzymatic and non-enzymatic synthesis of melanins, in Melanin: its role in human photoprotection (Zeiss, L., Chedekel, M. R. & Fitzpatrick, T. B., eds) pp. 109116, Valdenmar Publishing Co., Overland Park .
  • Quevedo, W. C., Fleischmann, R. D. & Dyckman, J. (1981) Premature loss of melanocytes from hair follicles of light (Blt) and silver (si) mice, in Pigment cell (Seiji, M., ed.) pp. 177183, University of Tokyo Press, Tokyo .
  • Quevedo, W. C. Jr & Chase, H. B. (1958) An analysis of the light mutation of coat color in mice, J. Morphol. 102, 329346.
  • Salinas, C., Garcia-Borron, J. C., Solano, F. & Lozano, J. A. (1994) Dopachrome tautomerase decreases the binding of indolic melanogenesis intermediates to proteins, Biochim. Biophys. Acta 1204, 5360.
  • Schaumburg-Lever, G., Metzler, G. & Kaiserling, E. (1991) Ultrastructural localization of HMB-45 binding sites, J. Cutaneous Pathol. 18, 432435.
  • Silvers, W. K. (1979) Beige, Silver, Graying with Age, and other Determinants, pp. 134136, in The coat colors of mice: a model for mammalian gene action and interaction, Springer-Verlag, New York .
  • Spanakis, E., Lamina, P. & Bennett, D. C. (1992) Effects of the developmental colour mutations silver and recessive spotting on proliferation of diploid and immortal mouse melanocytes in culture, Development (Camb.) 114, 675680.
  • Taatjes, D. J., Arendash-Durand, B., von Turkovich, M. & Trainer, T. D. (1993) HMB-45 antibody demonstrates melanosome specificity by immunoelectron microscopy, Arch. Pathol. Lab. Med. 117, 264268.
  • Tomita, Y., Hariu, A., Mizuno, C. & Seiji, M. (1980) Inactivation of tyrosinase by dopa, J. Invest. Dermatol. 75, 379382.
  • Tomita, Y., Hariu, A., Kato, C. & Seiji, M. (1984) Radical production during tyrosinase reaction, dopa-melanin formation, and photoirradiation of dopamelanin, J. Invest. Dermatol. 82, 573576.
  • Tsukamoto, K., Palumbo, A., D Ischia, M., Hearing, V. J. & Proti, G. (1992) 5,6–Dihydroxyindole-2-carboxylic acid is incorporated in mammalian melanin, Biochem. J. 286, 491495.
  • Vennegoor, C., Hagerman, P., Van Nouhuijs, H., Ruiter, D. J., Calafat, J., Ringens, P. J. & Rumke, P. (1988) A monoclonal antibody specific for cells of the melanocyte lineage, Am. J. Pathol. 130, 179192.
  • Wick, M. R., Swanson, P. E. & Rocamora, A. (1988) Recognition of malignant melanoma by monoclonal antibody HMB-45. An immunohistochemical study of 200 paraffin-embedded cutaneous tumors, J. Cutaneous Pathol. 15, 201207.
  • Zhou, B.-K., Kobayashi, T., Donatien, P. D., Bennett, D. C., Hearing, V. J. & Orlow, S. J. (1994) Identification of a melanosomal matrix protein encoded by the murine si (silver) locus using ‘organelle Scanning’, Proc. Natl. Acad. Sci. USA 91, 70767080.