A Heterodimer of the Zn2Cys6 Transcription Factors Pip2p and Oaf1p Controls Induction of Genes Encoding Peroxisomal Proteins in Saccharomyces Cerevisiae

Authors

  • Hanspeter Rottensteiner,

    1. Vienna Biocenter, Institut für Biochemie und Molekulare Zellbiologie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, Wien, Austria
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  • Arnoud J. Kal,

    1. Department of Biochemistry, Academic Medical Center of the Universit y of Amsterdam, Amsterdam, The Netherlands
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  • Barbara Hamilton,

    1. Vienna Biocenter, Institut für Biochemie und Molekulare Zellbiologie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, Wien, Austria
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  • Helmut Ruis,

    1. Vienna Biocenter, Institut für Biochemie und Molekulare Zellbiologie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, Wien, Austria
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  • Henk F. Tabak

    Corresponding author
    1. Department of Biochemistry, Academic Medical Center of the Universit y of Amsterdam, Amsterdam, The Netherlands
      H. F. Tabak, Department of Biochemistry, Academic Medical Center of the University of Amsterdam, Meibergdreef 15, NL-1105 AZ Amsterdam, The Netherlands
      Fax:+31 20 6915519.
      E-mail:h.f.tabak@amc.uva.nl
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  • Note. H. Rottensteiner and A. J. Kal contributed equally to this paper.

H. F. Tabak, Department of Biochemistry, Academic Medical Center of the University of Amsterdam, Meibergdreef 15, NL-1105 AZ Amsterdam, The Netherlands
Fax:+31 20 6915519.
E-mail:h.f.tabak@amc.uva.nl

Abstract

In the yeast Saccharornyces cerevisiae, two transcriptional activators belonging to the Zn2Cys6 protein family, Pip2p and Oaf1p, are involved in fatty-acid-dependent induction of genes encoding peroxisomal proteins. This induction is mediated via an upstream activation sequence called the oleate-response element (ORE). DNA-bandshift experiments with ORE probes and epitope-tagged proteins showed that two binary complexes occurred: in wild-type cells the major complex consisted of a Pip2p · Oaf1p heterodimer, but in cells in which Oaf1p was overexpressed an Oaf1p homodimer was also observed. The genes encoding Oaf1p and Pip2p were controlled in different ways. The OAF1 gene was constitutively expressed, while the PIP2 gene was induced upon growth on oleate, giving rise to positive autoregulatory control. We have shown that the Pip2p · Oaf1p heterodimer is responsible for the strong expression of the genes encoding peroxisomal proteins upon growth on oleate. Pip2p and Oaf1p form an example of a heterodimere of yeast Zn2Cys6 zinc-finger proteins binding to DNA.

Abbreviations.
HA

haemagglutinin epitope

GST

glutathione S-transferase

ORE

oleate-response element

PPAR

peroxisome proliferator-activated receptor

Ancillary

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