Note. No distinctions are made between CO2, H2CO3, HCO3−, and Co32− when the symbol ‘CO2’ is used.
The Active Species of ‘CO2’ Utilized by Formylmethanofuran Dehydrogenase from Methanogenic Archaea
Article first published online: 16 JUL 2004
European Journal of Biochemistry
Volume 248, Issue 3, pages 919–924, September 1997
How to Cite
Vorholt, J. A. and Thauer, R. K. (1997), The Active Species of ‘CO2’ Utilized by Formylmethanofuran Dehydrogenase from Methanogenic Archaea. European Journal of Biochemistry, 248: 919–924. doi: 10.1111/j.1432-1033.1997.00919.x
- Issue published online: 16 JUL 2004
- Article first published online: 16 JUL 2004
- (Received 23 June 1997) – EJB 97 0899/4
- methanogenic Archaea;
- Methanosarcina barkeri;
- methanogenesis from CO2;
- formylmethano furan dehydrogenase;
Formylmethanofuran dehydrogenase from methanogenic Archaea catalyzes the reversible conversion of CO2 and methanofuran to formylmethanofuran, which is an intermediate in methanogenesis from CO2 a biological process yielding approximately 0.3 billion tons of CH4 per year. With the enzyme from Methanosarcina barkeri, it is shown that CO2 rather than HCO3 is the active species of ‘CO2’ utilized by the dehydrogenase. Evidence is also presented that the enzyme catalyzes a methanofuran-dependent exchange between CO2 and the formyl group of formylmethanofuran. The results are consistent with N-carboxymethanofuran being an intermediate in CO2 reduction to formylmethanofuran.