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Anthocyanin 5-Aromatic Acyltransferase from Gentiana Triflora

Purification, Characterization and its Role in Anthocyanin Biosynthesis

Authors


H. Fujiwara, Institute for Fundamental Research, Suntory Ltd, 1–1–1, Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka, Japan 618
Fax:+ 81 75 962 8262.
E-mail:src08807@nisiq.net

Abstract

Acylation with hydroxycinnamic acids stabilizes anthocyanins and makes their colour bluer (bathochromic shift). We purified to homogeneity one acylation enzyme, hydroxycinnamoyl-CoA:anthocyanidin 3,5-diglucoside 5-O-glucoside-6′″-O-hydroxycinnamoyltransferase, from blue petals of Gentiana triflora. It is a single polypeptide protein of 52 kDa with a pi of 4.6. It catalyzes the transfer of the p-coumaric acid and caffeic acid from their CoA esters to the 5-glucosyl moiety of anthocyanidin 3,5-diglucosides but could not use malonyl-CoA as an acyl donor. Neither anthocyanidin 3-monoglucoside nor anthocyanins aromatically acylated at the 3-glucosyl moiety could be acylated by this enzyme. Aromatic acylation of anthocyanidin 3,5-diglucoside by this enzyme caused a bathochromic shift and increased pigment stability in neutral to weakly basic pH. Other anthocyanins from the petals of G. triflora were isolated and their structures were determined by fast-atom-bombardment MS and NMR. The biosynthetic pathway of genti-odelphin, a diacylated anthocyanin accumulating in G. triflora petals, is proposed on the basis of these results.

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