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Purification and Characterization of Transcription Factor IIIA from Higher Plants

Authors

  • Eliza Wyszko,

    1. Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego, Poznań, Poland
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  • Mirostawa Barciszewska

    Corresponding author
    1. Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego, Poznań, Poland
      M. Barciszewska, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, PL-61704, Poznań, Poland
      Fax: + 48 61 852 05 32.
      E-mail: mbarcisz@ibch.poznan.pl
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M. Barciszewska, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, PL-61704, Poznań, Poland
Fax: + 48 61 852 05 32.
E-mail: mbarcisz@ibch.poznan.pl

Abstract

Transcription factor IIIA (TF IIIA) binds and specifically activates transcription of eukaryotic 5S rRNA genes. It also forms a 7S ribonucleoprotein complex with mature 5S rRNA. Here, we describe the purification and properties of pTF IIIA from higher plants. The purified protein from tulip (Tulipa whittalii) has a molecular mass of about 40 kDa and also binds 5S rRNA and 5S rRNA genes. pTF IIIA also facilitates the transcription of a 5S rRNA gene in a HeLa cell extract.

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