Alterations of glucocorticoid receptor expression during glucocorticoid hormone therapy in renal transplant patients

Authors


E-mail: timea.berki@aok.pte.hu Tel.: +36-72-536291 Fax: +36-72-536289

Abstract

Abstract Previous studies have demonstrated that glucocorticoid resistance develops in some patients during glucocorticoid therapy due to the down-regulation of glucocorticoid receptor (GCR) expression. A new flow cytometric method has been used for monitoring the intracellular GCR level in peripheral blood mononuclear cells (PBMCs). GCR expression by different lymphocyte subpopulations (bearing surface CD4+ CD8+ or CD19+) in steroid-treated (ST) and nontreated (NT) renal transplant patients was also compared. High, decreasing-dose steroid treatment caused a reduced GCR expression in 50% of the renal transplant patients. Long-lasting, low-dose steroid therapy caused a fall in GCR level in only 11% of patients. CD4+ T lymphocytes showed both the lowest GCR level and its alteration due to steroid therapy, while CD8+ T cells and CD19+ B lymphocytes expressed higher GCR levels and were more sensitive to steroid treatment. Detection of GCR expression in lymphocytes may provide useful information about the changes of glucocorticoid senstivity during the therapy.

Abbreviation
FITC

fluorescein isothiocyanate

GC

glucocorticoid hormone

GCR

glucocorticoid receptor

mAb

monoclonal antibody

NT

non-treated

PBMCs

peripheral blood mononuclear cells

PBS

phosphate-buffered saline

PE

phycoerythrin

PFA

paraformaldehyde

ST

steroid-treated

Ancillary