ORIGINAL ARTICLE

Improved GFR and renal plasma perfusion following remote ischaemic conditioning in a porcine kidney transplantation model

  1. Peter Soendergaard1,†,
  2. Nicoline V. Krogstrup1,†,
  3. Niels G. Secher4,
  4. Kristian Ravlo1,
  5. Anna K. Keller5,
  6. Else Toennesen4,
  7. Bo M. Bibby6,
  8. Ulla Moldrup2,
  9. Ernst O. Ostraat2,
  10. Michael Pedersen3,5,
  11. Troels M. Jorgensen2,
  12. Henri Leuvenink7,
  13. Rikke Norregaard5,
  14. Henrik Birn1,
  15. Niels Marcussen8,
  16. Bente Jespersen1

Article first published online: 6 JUL 2012

DOI: 10.1111/j.1432-2277.2012.01522.x

Issue

Transplant International

Transplant International

Volume 25, Issue 9, pages 1002–1012, September 2012

Additional Information

How to Cite

Soendergaard, P., Krogstrup, N. V., Secher, N. G., Ravlo, K., Keller, A. K., Toennesen, E., Bibby, B. M., Moldrup, U., Ostraat, E. O., Pedersen, M., Jorgensen, T. M., Leuvenink, H., Norregaard, R., Birn, H., Marcussen, N. and Jespersen, B. (2012), Improved GFR and renal plasma perfusion following remote ischaemic conditioning in a porcine kidney transplantation model. Transplant International, 25: 1002–1012. doi: 10.1111/j.1432-2277.2012.01522.x

Author Information

  1. 1

     Department of Renal Medicine, Aarhus University Hospital, Aarhus, Denmark

  2. 2

     Department of Urology, Aarhus University Hospital, Aarhus, Denmark

  3. 3

     MR Research Center, Aarhus University Hospital, Aarhus, Denmark

  4. 4

     Department of Anaesthesiology and Intensive Care, Aarhus University Hospital, Aarhus, Denmark

  5. 5

     Department of Clinical Medicine, Aarhus University, Aarhus, Denmark

  6. 6

     Department of Biostatistics, Aarhus University, Aarhus, Denmark

  7. 7

      Department of Surgery, University of Groningen, Groningen, the Netherlands

  8. 8

      Department of Pathology, Odense University Hospital, Odense, Denmark

  1. †

    Both authors contributed equally to this work.

*Nicoline V. Krogstrup MD, Department of Renal Medicine, Aarhus University Hospital, Brendstrupgaardsvej100, 8200 Aarhus N, Denmark. Tel.: +45 78 45 24 17; fax: +45 78 45 24 30; e-mail: nicoline.v.krogstrup@ki.au.dk

  1. Conflicts of Interest No conflicts of interest.

  2. †

    Both authors contributed equally to this work.

Publication History

  1. Issue published online: 6 AUG 2012
  2. Article first published online: 6 JUL 2012
  3. Received: 14 February 2012 Revision requested: 23 March 2012Accepted: 4 June 2012 Published online: 6 July 2012

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (291K)

Keywords:

  • glomerular filtration rate;
  • ischaemic conditioning;
  • kidney transplantation;
  • neutrophil gelatinase-associated lipocalin;
  • renal plasma perfusion

Summary

Delayed graft function (DGF) complicates approximately 25% of kidney allografts donated after brain death (DBD). Remote ischaemic conditioning (rIC) involves brief, repetitive, ischaemia in a distant tissue in connection with ischaemia/reperfusion in the target organ. rIC has been shown to induce systemic protection against ischaemic injuries. Using a porcine kidney transplantation model with donor (63 kg) recipient (15 kg) size mismatch, we investigated the effects of recipient rIC on early renal plasma perfusion and GFR. Brain death was induced in donor pigs (n = 8) and kidneys were removed and kept in cold storage until transplantation. Nephrectomized recipient pigs were randomized to rIC (n = 8) or non-rIC (n = 8) with one kidney from the same donor in each group. rIC consisted of 4 × 5 min clamping of the abdominal aorta. GFR was significantly higher in the rIC group compared with non-rIC (7.2 ml/min vs. 3.4 ml/min; ΔGFR = 3.7 ml/min, 95%-CI: 0.3–7.2 ml/min, P = 0.038). Renal plasma perfusion in both cortex and medulla measured by dynamic contrast-enhanced magnetic resonance imaging (MRI) was significantly higher over time in the rIC group compared with non-rIC. This experimental study demonstrated a positive effect of rIC on early graft perfusion and function in a large animal transplantation model.

View Full Article (HTML) Get PDF (291K)