Dedicated to Prof. Dr. Schnepf on the occasion of his 65 th birthday.
Inhibition of Intracellular Pectin Transport in Pollen Tubes by Monensin, Brefeldin A and Cytochalasin D*
Version of Record online: 27 FEB 2014
1996 Deutsche Botanische Gesellschaft/German Botanical Society
Volume 109, Issue 5, pages 373–381, October 1996
How to Cite
Geitmann, A., Wojciechowicz, K. and Cresti, M. (1996), Inhibition of Intracellular Pectin Transport in Pollen Tubes by Monensin, Brefeldin A and Cytochalasin D. Botanica Acta, 109: 373–381. doi: 10.1111/j.1438-8677.1996.tb00586.x
- Issue online: 27 FEB 2014
- Version of Record online: 27 FEB 2014
- Received: November 2, 1995; Accepted: January 30, 1996
- Brefeldin A;
- cytochalasin D;
- Nicotiana tabacum;
- pollen tube
Specific inhibitors of the secretory pathway represent important tools for investigation of cell wall synthesis and tip growth in pollen tubes. Brefeldin A completely inhibits germination of Nicotiana tabacum pollen tubes at 2.2 μM. Ultrastructural investigation of pollen tube cytoplasm showed that brefeldin A caused the appearance of reticular structures and “brefeldin A compartments” containing unesterified pectins. Monensin caused inhibition of pollen tube germination at 80 nM. The drug induced swelling of the Golgi cisternae, many of which contained methyl-esterified pectins. Cytochalasin D was effective at 1 μg/ml, the inhibition of germination being fully reversible. Application of the drug caused accumulation of secretory vesicles containing methyl-esterified pectin around the dictyosomes. In contrast to brefeldin A and monensin, cytochalasin D caused a slowdown of cytoplasmic streaming. Monensin, but not the other drugs, caused a considerable decrease in pollen tube diameter. The characterization and quantification of the effects of the drugs on pollen tubes represents a necessary prerequisite for their application in physiological studies.