These authors contributed equally to the results of this study.
Differential transcriptional expression following thidiazuron-induced callus differentiation developmental shifts in rice
Article first published online: 2 JUN 2009
© 2009 National Botanical Research Institute, CSIR
Volume 12, Issue 1, pages 46–59, January 2010
How to Cite
Chakrabarty, D., Trivedi, P. K., Shri, M., Misra, P., Asif, M. H., Dubey, S., Kumar, S., Rai, A., Tiwari, M., Shukla, D., Pandey, A., Nigam, D., Tripathi, R. D. and Tuli, R. (2010), Differential transcriptional expression following thidiazuron-induced callus differentiation developmental shifts in rice. Plant Biology, 12: 46–59. doi: 10.1111/j.1438-8677.2009.00213.x
Editor S. Wick
- Issue published online: 8 DEC 2009
- Article first published online: 2 JUN 2009
- Received: 3 January 2009; Accepted: 14 March 2009
- shoot meristem;
Very little is known about molecular events associated with callus differentiation in indica rice. The genes expressed differentially during shoot meristem initiation were identified on genomic arrays applied to efficiently regenerating rice calli. A thidiazuron (TDZ; N-phenyl-N-thiadiazol-1,2,3–5,ylurea)-dependent regeneration protocol was developed for efficient embryogenesis in indica rice. The regenerating embryogenic calli induced by TDZ for 10 days showed transcriptional modulation of a number of genes associated with photosynthesis, hormone metabolism, plant development, signal transduction, light response, and plant defense. Eighteen candidate miRNAs were predicted to target the genes expressed differentially in the embryogenic calli grown in TDZ-containing medium. The majority of the photosynthesis-related genes up-regulated in differentiating calli were not expressed or were down-regulated in developing seeds and inflorescences. Most of the genes down-regulated in differentiating calli were up-regulated in developing seeds. The transcriptome of differentiating callus most closely resembled that of the germinating whole seed.