Targeted integration and removal of transgenes in hybrid aspen (Populus tremula L. × P. tremuloides Michx.) using site-specific recombination systems

Authors

  • Matthias Fladung,

    1.  Johann Heinrich von Thünen-Institut, Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute of Forest Genetics, Grosshansdorf, Germany
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  • Dirk Becker

    1.  Biocentre Klein Flottbek, Developmental Biology and Biotechnology, University of Hamburg, Hamburg, Germany
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Errata

This article corrects:

  1. Targeted integration and removal of transgenes in hybrid aspen (Populus tremula L. x P. tremuloides Michx.) using site-specific recombination systems Volume 12, Issue 2, 334–340, Article first published online: 23 December 2009

Matthias Fladung, Johann Heinrich von Thünen-Institut, Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute of Forest Genetics, Grosshansdorf, Germany. Email: matthias.fladung@vti.bund.de

In Fig. 8 of the study, we suggested a strategy for targeted transgene transfer and, at the same time, removal of antibiotic marker genes. Unfortunately, a small mistake has been slipped in under (B) of this figure in “2. Recombination = induction of cre = removal of bar/hyg/cre” (Fladung & Becker 2010). The FRT site (stumped blue arrow) located at the 3′-end of the ‘gene-of-interest’ in the starting cassette has moved to the 5′-end of the ‘gene-of-interest’ in the next drawing. The correct position of the FRT site, however, is still at the 3′-end of the ‘gene-of-interest’ as shown in the corrected Fig. 8.

Figure 8.

 Suggested strategy for targeted transgene transfer and, at the same time, removal of antibiotic marker genes.

The consequence of the mistake is that a putative third recombination by external applied FLP as mentioned in the text would NOT remove the additional FRT/Lox-fragment but rather would lead to the removal of the ‘gene-of-interest’. Therefore, no third recombination is recommended.

Corrected Figure 8:

Ancillary